Organization and Expression of the Mitochondrial Genome in the Nicotiana sylvestris CMSII Mutant

Organization and Expression of the Mitochondrial Genome in the Nicotiana sylvestris CMSII Mutant

Christine Lelandais^a, Béatrice Albert^a,b, Sophie Gutierres^a, Rosine De Paepe^a, Bernard Godelle^b, Fernand Vedel^a, and Philippe Chétrit^a
^a Institut de Biotechnologie des Plantes, Université Paris-Sud, 91405 Orsay Cedex, France
^b Laboratoire d'Evolution et Systématique, Université Paris-Sud, 91405 Orsay Cedex, France

Corresponding author: Rosine De Paepe, Institut de Biotechnologie des Plantes, Bât 630, Université Paris-Sud, 91405 Orsay Cedex, France., depaepe{at}sidonie.ibp.u-psud.fr (E-mail).

Communicating editor: K. J. NEWTON

Previous analyses suggested that the Nicotiana sylvestris CMSIImutant carried a large deletion in its mitochondrial genome.Here, we show by cosmid mapping that the deletion is 60 kb inlength and contains several mitochondrial genes or ORFs, includingthe complex I nad7 gene. However, due to the presence of largeduplications in the progenitor mitochondrial genome, the onlyunique gene that appears to be deleted is nad7. RNA gel blotdata confirm the absence of nad7 expression, strongly suggestingthat the molecular basis for the CMSII abnormal phenotype, poorgrowth and male sterility, is the altered complex I structure.The CMSII mitochondrial genome appears to consist essentiallyof one of two subgenomes resulting from recombination betweendirect short repeats. In the progenitor mitochondrial genomeboth recombination products are detected by PCR and, reciprocally,the parental fragments are detected at the substoichiometriclevel in the mutant. The CMSII mtDNA organization has been maintainedthrough six sexual generations.

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