Genetics, Vol. 150, 815-822, October 1998, Copyright © 1998

Deletion Mapping of the Head Tilt (het) Gene in Mice: A Vestibular Mutation Causing Specific Absence of Otoliths

Rebecca A. Bergstroma, Yun You, Lawrence C. Erwayb, Mary F. Lyonc, and John C. Schimentia
a The Jackson Laboratory, Bar Harbor, Maine 04609,
b Department of Biological Sciences, University of Cincinnati, Cincinnati, Ohio 45221
c MRC Mammalian Genetics Unit, Chilton, Didcot, Oxfordshire OX11 ORD, United Kingdom

Corresponding author: John C. Schimenti, The Jackson Laboratory, 600 Main St., Bar Harbor, ME 04660., jcs{at}jax.org (E-mail).

Communicating editor: N. A. JENKINS

Head tilt (het) is a recessive mutation in mice causing vestibular dysfunction. Homozygotes display abnormal responses to position change and linear acceleration and cannot swim. However, they are not deaf. het was mapped to the proximal region of mouse chromosome 17, near the T locus. Here we report anatomical characterization of het mutants and high resolution mapping using a set of chromosome deletions. The defect in het mutants is limited to the utricle and saccule of the inner ear, which completely lack otoliths. The unique specificity of the het mutation provides an opportunity to better understand the development of the vestibular system. Complementation analyses with a collection of embryonic stem (ES)- and germ cell-induced deletions localized het to an interval near the centromere of chromosome 17 that was indivisible by recombination mapping. This approach demonstrates the utility of chromosome deletions as reagents for mapping and characterizing mutations, particularly in situations where recombinational mapping is inadequate.




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