P-Element Insertion at the polyhomeotic Gene Leads to Formation of a Novel Chimeric Protein That Negatively Regulates yellow Gene Expression in P-Element-Induced Alleles of Drosophila melanogaster

P-Element Insertion at the polyhomeotic Gene Leads to Formation of a Novel Chimeric Protein That Negatively Regulates yellow Gene Expression in P-Element-Induced Alleles of Drosophila melanogaster

Tatiana Belenkaya^a,b, Alexey Soldatov^a,b,c, Elena Nabirochkina^a,c, Inna Birjukova^a, Sofia Georgieva^a,b,c, and Pavel Georgiev^a,c
^a Department of the Control of Genetic Processes, Russian Academy of Sciences, Moscow 117334, Russia,
^b Unit of Oslo University, Institute of Gene Biology, Russian Academy of Sciences, Moscow 117334, Russia
^c International Centre of Genetic Engineering and Biotechnology, 34012 Trieste, Italy

Corresponding author: Pavel Georgiev, Institute of Gene Biology, Russian Academy of Sciences, 34/5 Vavilov St., Moscow 117334, Russia., pgeorg{at}biogen.msk.su (E-mail).

Communicating editor: J. A. BIRCHLER

Polyhomeotic is a member of the Polycomb group (Pc-G) of homeoticrepressors. The proteins encoded by the Pc-G genes form repressivecomplexes on the polycomb group response element sites. Theph^P1 mutation was induced by insertion of a 1.2-kb P elementinto the 5' transcribed nontranslated region of the proximalpolyhomeotic gene. The ph^P1 allele confers no mutant phenotype,but represses transcription of P-element-induced alleles atthe yellow locus. The ph^P1 allele encodes a chimeric P-PH protein,consisting of the DNA-binding domain of the P element and thePH protein lacking 12 amino-terminal amino acids. The P-PH,Polycomb (PC), and Posterior sex combs (PSC) proteins were immunohistochemicallydetected on polytene chromosomes in the regions of P-elementinsertions.

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