Regulation of Gene Expression During the Vegetative Incompatibility Reaction in Podospora anserina: Characterization of Three Induced Genes

Corresponding author: Corinne Clavé, Laboratoire de Génétique Moléculaire des Champignons, Institut de Biochimie et de Génétique Cellulaires, UPR CNRS 9026, 1, rue Camille Saint-Saëns, 33077 Bordeaux cedex, France., corinne.clave{at}ibgc.u-bordeaux2.fr (E-mail).

Communicating editor: R. H. DAVIS

Vegetative incompatibility in fungi limits the formation of viable heterokaryons. It results from the coexpression of incompatible genes in the heterokaryotic cells and leads to a cell death reaction. In Podospora anserina, a modification of gene expression takes place during this reaction, including a strong decrease of total RNA synthesis and the appearance of a new set of proteins. Using in vitro translation of mRNA and separation of protein products by two-dimensional gel electrophoresis, we have shown that the mRNA content of cells is qualitatively modified during the progress of the incompatibility reaction. Thus, gene expression during vegetative incompatibility is regulated, at least in part, by variation of the mRNA content of specific genes. A subtractive cDNA library enriched in sequences preferentially expressed during incompatibility was constructed. This library was used to identify genomic loci corresponding to genes whose mRNA is induced during incompatibility. Three such genes were characterized and named idi genes for genes induced during incompatibility. Their expression profiles suggest that they may be involved in different steps of the incompatibility reaction. The putative IDI proteins encoded by these genes are small proteins with signal peptides. IDI-2 protein is a cysteine-rich protein. IDI-2 and IDI-3 proteins display some similarity in a tryptophan-rich region.

This article has been cited by other articles:

				K. Pal, A.D. van Diepeningen, J. Varga, R.F. Hoekstra, P.S. Dyer, and A.J.M. Debets Sexual and vegetative compatibility genes in the aspergilli. Stud Mycol, January 1, 2007; 59: 19 - 30. [Abstract] [Full Text] [PDF]

				K. Dementhon, G. Iyer, and N. L. Glass VIB-1 Is Required for Expression of Genes Necessary for Programmed Cell Death in Neurospora crassa Eukaryot. Cell, December 1, 2006; 5(12): 2161 - 2173. [Abstract] [Full Text] [PDF]

				B. Pinan-Lucarre, A. Balguerie, and C. Clave Accelerated Cell Death in Podospora Autophagy Mutants Eukaryot. Cell, November 1, 2005; 4(11): 1765 - 1774. [Abstract] [Full Text] [PDF]

				K. Dementhon, M. Paoletti, B. Pinan-Lucarre, N. Loubradou-Bourges, M. Sabourin, S. J. Saupe, and C. Clave Rapamycin Mimics the Incompatibility Reaction in the Fungus Podospora anserina Eukaryot. Cell, April 1, 2003; 2(2): 238 - 246. [Abstract] [Full Text] [PDF]

				N. L. Glass and I. Kaneko Fatal Attraction: Nonself Recognition and Heterokaryon Incompatibility in Filamentous Fungi Eukaryot. Cell, February 1, 2003; 2(1): 1 - 8. [Full Text] [PDF]

				S. J. Saupe Molecular Genetics of Heterokaryon Incompatibility in Filamentous Ascomycetes Microbiol. Mol. Biol. Rev., September 1, 2000; 64(3): 489 - 502. [Abstract] [Full Text] [PDF]