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Genetics, Vol. 150, 75-93, September 1998, Copyright © 1998

The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 Genes Are Required for Tolerating Irreparable, Ultraviolet-Induced DNA Damage

A. G. Paulovicha, C. D. Armoura, and L. H. Hartwella
a Fred Hutchinson Cancer Research Center, Seattle, Washington 98109

Corresponding author: L. H. Hartwell, Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., LY301, Seattle, WA 98109-1024., lhartwel{at}fhcrc.org (E-mail).

Communicating editor: M. D. ROSE

In wild-type Saccharomyces cerevisiae, a checkpoint slows the rate of progression of an ongoing S phase in response to exposure to a DNA-alkylating agent. Mutations that eliminate S phase regulation also confer sensitivity to alkylating agents, leading us to suggest that, by regulating the S phase rate, cells are either better able to repair or better able to replicate damaged DNA. In this study, we determine the effects of mutations that impair S phase regulation on the ability of excision repair-defective cells to replicate irreparably UV-damaged DNA. We assay survival after UV irradiation, as well as the genetic consequences of replicating a damaged template, namely mutation and sister chromatid exchange induction. We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 (but not REV3, RAD24, and MEC3) are required for maximal induction of replication-dependent sister chromatid exchange. Therefore, checkpoint genes not only control cell cycle progression in response to damage, but also play a role in accommodating DNA damage during replication.




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