help button home button Genetics AJP: Advances PE
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Friesen, H.
Right arrow Articles by Segall, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Friesen, H.
Right arrow Articles by Segall, J.
Genetics, Vol. 150, 59-73, September 1998, Copyright © 1998

SPE3, Which Encodes Spermidine Synthase, Is Required for Full Repression Through NREDIT in Saccharomyces cerevisiae

Helena Friesena, Jason C. Tannyb, and Jacqueline Segalla,b
a Department of Biochemistry, University of Toronto, Toronto, Ontario M5S 1A8, Canada
b Department of Molecular and Medical Genetics, University of Toronto, Toronto, Ontario M5S 1A8, Canada

Corresponding author: Jacqueline Segall, Department of Biochemistry, University of Toronto, Toronto, ON M5S 1A8 Canada., j.segall{at}utoronto.ca (E-mail).

Communicating editor: F. WINSTON

We previously identified a transcriptional regulatory element, which we call NREDIT, that is required for repression of the sporulation-specific genes, DIT1 and DIT2, during vegetative growth of Saccharomyces cerevisiae. Repression through this element is dependent on the Ssn6-Tup1 corepressor. In this study, we show that SIN4 contributes to NREDIT-mediated repression, suggesting that changes in chromatin structure are, at least in part, responsible for regulation of DIT gene expression. In a screen for additional genes that function in repression of DIT (FRD genes), we recovered alleles of TUP1, SSN6, SIN4, and ROX3 and identified mutations comprising eight complementation groups of FRD genes. Four of these FRD genes appeared to act specifically in NREDIT-mediated repression, and four appeared to be general regulators of gene expression. We cloned the gene complementing the frd3-1 phenotype and found that it was identical to SPE3, which encodes spermidine synthase. Mutant spe3 cells not only failed to support complete repression through NREDIT but also had modest defects in repression of some other genes. Addition of spermidine to the medium partially restored repression to spe3 cells, indicating that spermidine may play a role in vivo as a modulator of gene expression. We suggest various mechanisms by which spermidine could act to repress gene expression.




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
K. Rothfels, J. C. Tanny, E. Molnar, H. Friesen, C. Commisso, and J. Segall
Components of the ESCRT Pathway, DFG16, and YGR122w Are Required for Rim101 To Act as a Corepressor with Nrg1 at the Negative Regulatory Element of the DIT1 Gene of Saccharomyces cerevisiae
Mol. Cell. Biol., August 1, 2005; 25(15): 6772 - 6788.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. C. Howard, Y.-W. Chang, Y. V. Budovskaya, and P. K. Herman
The Ras/PKA Signaling Pathway of Saccharomyces cerevisiae Exhibits a Functional Interaction With the Sin4p Complex of the RNA Polymerase II Holoenzyme
Genetics, September 1, 2001; 159(1): 77 - 89.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Yu, P. Eriksson, and D. J. Stillman
Architectural Transcription Factors and the SAGA Complex Function in Parallel Pathways To Activate Transcription
Mol. Cell. Biol., April 1, 2000; 20(7): 2350 - 2357.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Genetics Society of America.