Genetics, Vol. 149, 1729-1737, August 1998, Copyright © 1998

The Schizosaccharomyces pombe S-Phase Checkpoint Differentiates Between Different Types of DNA Damage

Nicholas Rhinda and Paul Russella
a Departments of Molecular Biology and Cell Biology, The Scripps Research Institute, La Jolla, California 92037

Corresponding author: Paul Russell, The Scripps Research Institute, MB3, 10550 North Torrey Pines Rd., La Jolla, CA 92037., prussell{at}scripps.edu (E-mail).

Communicating editor: G. R. SMITH

We have identified an S-phase DNA damage checkpoint in Schizosaccharomyces pombe. This checkpoint is dependent on Rad3, the S. pombe homolog of the mammalian ATM/ATR checkpoint proteins, and Cds1. Cds1 had previously been believed to be involved only in the replication checkpoint. The requirement of Cds1 in the DNA damage checkpoint suggests that Cds1 may be a general target of S-phase checkpoints. Unlike other checkpoints, the S. pombe S-phase DNA damage checkpoint discriminates between different types of damage. UV-irradiation, which causes base modification that can be repaired during G1 and S-phase, invokes the checkpoint, while {gamma}-irradiation, which causes double-stranded breaks that cannot be repaired by a haploid cell if induced before replication, does not invoke the checkpoint. Because the same genes are required to respond to UV- and {gamma}-irradiation during G2, this discrimination may represent an active suppression of the {gamma} response during S-phase.




This article has been cited by other articles:


Home page
 Mol. Biol. CellHome page
N. Willis and N. Rhind
Mus81, Rhp51(Rad51), and Rqh1 Form an Epistatic Pathway Required for the S-Phase DNA Damage Checkpoint
Mol. Biol. Cell, February 1, 2009; 20(3): 819 - 833.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
H. Diaz-Cuervo and A. Bueno
Cds1 Controls the Release of Cdc14-like Phosphatase Flp1 from the Nucleolus to Drive Full Activation of the Checkpoint Response to Replication Stress in Fission Yeast
Mol. Biol. Cell, June 1, 2008; 19(6): 2488 - 2499.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. J. Callegari and T. J. Kelly
From the Cover: UV irradiation induces a postreplication DNA damage checkpoint
PNAS, October 24, 2006; 103(43): 15877 - 15882.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
M. R. v. Z. K. Fagundes, C. P. Semighini, I. Malavazi, M. Savoldi, J. F. de Lima, M. H. de Souza Goldman, S. D. Harris, and G. H. Goldman
Aspergillus nidulans uvsBATR and scaANBS1 Genes Show Genetic Interactions during Recovery from Replication Stress and DNA Damage
Eukaryot. Cell, July 1, 2005; 4(7): 1239 - 1252.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
D. G. Pankratz and S. L. Forsburg
Meiotic S-Phase Damage Activates Recombination without Checkpoint Arrest
Mol. Biol. Cell, April 1, 2005; 16(4): 1651 - 1660.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. A. Nilssen, M. Synnes, N. Kleckner, B. Grallert, and E. Boye
Intra-G1 arrest in response to UV irradiation in fission yeast
PNAS, September 16, 2003; 100(19): 10758 - 10763.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. Chahwan, T. M. Nakamura, S. Sivakumar, P. Russell, and N. Rhind
The Fission Yeast Rad32 (Mre11)-Rad50-Nbs1 Complex Is Required for the S-Phase DNA Damage Checkpoint
Mol. Cell. Biol., September 15, 2003; 23(18): 6564 - 6573.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
F. Zolezzi, J. Fuss, S. Uzawa, and S. Linn
Characterization of a Schizosaccharomyces pombe Strain Deleted for a Sequence Homologue of the Human Damaged DNA Binding 1 (DDB1) Gene
J. Biol. Chem., October 18, 2002; 277(43): 41183 - 41191.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
Y. Daniely, D. D. Dimitrova, and J. A. Borowiec
Stress-Dependent Nucleolin Mobilization Mediated by p53-Nucleolin Complex Formation
Mol. Cell. Biol., August 15, 2002; 22(16): 6014 - 6022.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
M. A. Marchetti, S. Kumar, E. Hartsuiker, M. Maftahi, A. M. Carr, G. A. Freyer, W. C. Burhans, and J. A. Huberman
A single unbranched S-phase DNA damage and replication fork blockage checkpoint pathway
PNAS, May 28, 2002; 99(11): 7472 - 7477.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
F. Osman, I. R. Tsaneva, M. C. Whitby, and C. L. Doe
UV Irradiation Causes the Loss of Viable Mitotic Recombinants in Schizosaccharomyces pombe Cells Lacking the G2/M DNA Damage Checkpoint
Genetics, March 1, 2002; 160(3): 891 - 908.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W. Feng and G. D'Urso
Schizosaccharomyces pombe Cells Lacking the Amino-Terminal Catalytic Domains of DNA Polymerase Epsilon Are Viable but Require the DNA Damage Checkpoint Control
Mol. Cell. Biol., July 15, 2001; 21(14): 4495 - 4504.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Kai, H. Tanaka, and T. S.-F. Wang
Fission Yeast Rad17 Associates with Chromatin in Response to Aberrant Genomic Structures
Mol. Cell. Biol., May 15, 2001; 21(10): 3289 - 3301.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
K. Tanaka, M. N. Boddy, X.-B. Chen, C. H. McGowan, and P. Russell
Threonine-11, Phosphorylated by Rad3 and ATM In Vitro, Is Required for Activation of Fission Yeast Checkpoint Kinase Cds1
Mol. Cell. Biol., May 15, 2001; 21(10): 3398 - 3404.
[Abstract] [Full Text]

Home page
 GeneticsHome page
E. J. Foss
Tof1p Regulates DNA Damage Responses During S Phase in Saccharomyces cerevisiae
Genetics, February 1, 2001; 157(2): 567 - 577.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
M. N. Boddy, A. Lopez-Girona, P. Shanahan, H. Interthal, W.-D. Heyer, and P. Russell
Damage Tolerance Protein Mus81 Associates with the FHA1 Domain of Checkpoint Kinase Cds1
Mol. Cell. Biol., December 1, 2000; 20(23): 8758 - 8766.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
M. Baum and L. Clarke
Fission Yeast Homologs of Human CENP-B Have Redundant Functions Affecting Cell Growth and Chromosome Segregation
Mol. Cell. Biol., April 15, 2000; 20(8): 2852 - 2864.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
P. U. Christensen, N. J. Bentley, R. G. Martinho, O. Nielsen, and A. M. Carr
Mik1 levels accumulate in S phase and may mediate an intrinsic link between S phase and mitosis
PNAS, March 14, 2000; 97(6): 2579 - 2584.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
C. Martín-Castellanos, M. A. Blanco, J. M. de Prada, and S. Moreno
The puc1 Cyclin Regulates the G1 Phase of the Fission Yeast Cell Cycle in Response to Cell Size
Mol. Biol. Cell, February 1, 2000; 11(2): 543 - 554.
[Abstract] [Full Text]

Home page
 J. Cell Sci.Home page
D Griffiths, M Uchiyama, P Nurse, and T. Wang
A novel mutant allele of the chromatin-bound fission yeast checkpoint protein Rad17 separates the DNA structure checkpoints
J. Cell Sci., January 3, 2000; 113(6): 1075 - 1088.
[Abstract] [PDF]

Home page
 Cold Spring Harb Symp Quant BiolHome page
J.-S. LEE, K. COLLINS, A. BROWN, C.-H. LEE, and J.H. CHUNG
The Function of BRCA1 in DNA Damage Response
Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 547 - 552.
[Abstract] [PDF]

Home page
 Mol. Cell. Biol.Home page
J.-M. Brondello, M. N. Boddy, B. Furnari, and P. Russell
Basis for the Checkpoint Signal Specificity That Regulates Chk1 and Cds1 Protein Kinases
Mol. Cell. Biol., June 1, 1999; 19(6): 4262 - 4269.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. L. Brown, C.-H. Lee, J. K. Schwarz, N. Mitiku, H. Piwnica-Worms, and J. H. Chung
A human Cds1-related kinase that functions downstream of ATM protein in the cellular response to DNA damage
PNAS, March 30, 1999; 96(7): 3745 - 3750.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Liang, J. Hodson, and S. Forsburg
Reduced dosage of a single fission yeast MCM protein causes genetic instability and S phase delay
J. Cell Sci., January 2, 1999; 112(4): 559 - 567.
[Abstract] [PDF]