Molecular Characterization of S Locus Genes, SLG and SRK, in a Pollen-Recessive Self-Incompatibility Haplotype of Brassica rapa L

Corresponding author: Katsunori Hatakeyama, Research Institute of Seed Production, Co., Ltd., 6-6-3, Minamiyoshinari, Aoba-ku, Sendai, 989-3204, Japan, hatake{at}tree.or.jp (E-mail).

Communicating editor: M. K. UYENOYAMA

In Brassica species that exhibit self-incompatibility, two genes, SLG and SRK, at the S locus are involved in the recognition reaction with self and non-self pollen. From a pollen-recessive S²⁹ haplotype of Brassica rapa, both cDNA and genomic DNA clones for these two genes were isolated and characterized. The nucleotide sequence for the S domain of SRK²⁹ showed a high degree of similarity with that of SLG²⁹, and they belong to Class II type. RNA gel blot analysis showed that the transcript of SLG²⁹ consisted of the first and second exons, and no other transcript containing any part of the intron sequence was detected. Because no transmembrane domain was encoded by the second exon of SLG²⁹, SLG²⁹ was designated a secreted type glycoprotein. SLGs of two other pollen-recessive haplotypes, S⁴⁰ and S⁴⁴, of B. rapa also had a similar structure to that of SLG²⁹. Previously, SLG² from a pollen-recessive haplotype, S², of Brassica oleracea was found to produce two different transcripts, one for the secreted type glycoprotein and the other for a putative membrane-anchored form of SLG. Therefore, the nature of these SLGs from pollen-recessive haplotypes of B. rapa is different from that of SLG² of B. oleracea.

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