Activation of Latent Transgenes in Arabidopsis Using a Hybrid Transcription Factor

Activation of Latent Transgenes in Arabidopsis Using a Hybrid Transcription Factor

Dave Guyer^a, Ann Tuttle^a, Sabrina Rouse^a, Sandra Volrath^a, Marie Johnson^a, Sharon Potter^a, Jörn Görlach^a, Steve Goff^a, Lyle Crossland^a, and Eric Ward^a
^a Novartis Agricultural Biotechnology Research, Research Triangle Park, North Carolina 27709

Corresponding author: Eric Ward, Novartis Agricultural Biotechnology Research, 3054 Cornwallis Road, P.O. Box 12257, Research Triangle Park, NC 27709, eric.ward{at}cp.novartis.com (E-mail).

Communicating editor: D. PREUSS

A hybrid transcription factor comprising a fusion of the DNA-bindingdomain of Saccharomyces cerevisiae GAL4 and the transcriptionactivation domain of maize C1 was expressed in stably transformedArabidopsis. Additional transgenic lines were created containingtest genes controlled by a synthetic promoter consisting ofconcatemeric copies of the cis-acting site recognized by GAL4(UAS_G) fused to a minimal promoter. The GAL4/C1 effector linewas crossed to two lines containing a synthetic promoter/GUSfusion. Both histochemical staining and GUS activity assaysindicate strong activation of GUS expression was achieved onlyafter crossing. The GAL4/C1 effector line was also crossed to15 lines containing a synthetic promoter/antisense adenylosuccinatesynthetase gene. Severely retarded growth, and in some caseslethality, was observed in 40% of the F₁ lines. This systemof activation by crossing is generally useful for activatingexpression of test transgenes.

This article has been cited by other articles:

B. C. Thomas, L. Rapaka, E. Lyons, B. Pedersen, and M. Freeling
Arabidopsis intragenomic conserved noncoding sequence
PNAS, February 27, 2007; 104(9): 3348 - 3353.
[Abstract] [Full Text] [PDF]

M. Imamura, J. Nakai, S. Inoue, G. X. Quan, T. Kanda, and T. Tamura
Targeted Gene Expression Using the GAL4/UAS System in the Silkworm Bombyx mori
Genetics, November 1, 2003; 165(3): 1329 - 1340.
[Abstract] [Full Text] [PDF]

J. Booker, S. Chatfield, and O. Leyser
Auxin Acts in Xylem-Associated or Medullary Cells to Mediate Apical Dominance
PLANT CELL, February 1, 2003; 15(2): 495 - 507.
[Abstract] [Full Text] [PDF]

P. Tornero, R. A. Chao, W. N. Luthin, S. A. Goff, and J. L. Dangl
Large-Scale Structure -Function Analysis of the Arabidopsis RPM1 Disease Resistance Protein
PLANT CELL, February 1, 2002; 14(2): 435 - 450.
[Abstract] [Full Text] [PDF]

G. J. Budziszewski, S. P. Lewis, L. W. Glover, J. Reineke, G. Jones, L. S. Ziemnik, J. Lonowski, B. Nyfeler, G. Aux, Q. Zhou, et al.
Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning
Genetics, December 1, 2001; 159(4): 1765 - 1778.
[Abstract] [Full Text] [PDF]

C. Boisnard-Lorig, A. Colon-Carmona, M. Bauch, S. Hodge, P. Doerner, E. Bancharel, C. Dumas, J. Haseloff, and F. Berger
Dynamic Analyses of the Expression of the HISTONE::YFP Fusion Protein in Arabidopsis Show That Syncytial Endosperm Is Divided in Mitotic Domains
PLANT CELL, March 1, 2001; 13(3): 495 - 509.
[Abstract] [Full Text]

				M. Imamura, J. Nakai, S. Inoue, G. X. Quan, T. Kanda, and T. Tamura Targeted Gene Expression Using the GAL4/UAS System in the Silkworm Bombyx mori Genetics, November 1, 2003; 165(3): 1329 - 1340. [Abstract] [Full Text] [PDF]

				J. Booker, S. Chatfield, and O. Leyser Auxin Acts in Xylem-Associated or Medullary Cells to Mediate Apical Dominance PLANT CELL, February 1, 2003; 15(2): 495 - 507. [Abstract] [Full Text] [PDF]

				P. Tornero, R. A. Chao, W. N. Luthin, S. A. Goff, and J. L. Dangl Large-Scale Structure -Function Analysis of the Arabidopsis RPM1 Disease Resistance Protein PLANT CELL, February 1, 2002; 14(2): 435 - 450. [Abstract] [Full Text] [PDF]

				G. J. Budziszewski, S. P. Lewis, L. W. Glover, J. Reineke, G. Jones, L. S. Ziemnik, J. Lonowski, B. Nyfeler, G. Aux, Q. Zhou, et al. Arabidopsis Genes Essential for Seedling Viability: Isolation of Insertional Mutants and Molecular Cloning Genetics, December 1, 2001; 159(4): 1765 - 1778. [Abstract] [Full Text] [PDF]

				C. Boisnard-Lorig, A. Colon-Carmona, M. Bauch, S. Hodge, P. Doerner, E. Bancharel, C. Dumas, J. Haseloff, and F. Berger Dynamic Analyses of the Expression of the HISTONE::YFP Fusion Protein in Arabidopsis Show That Syncytial Endosperm Is Divided in Mitotic Domains PLANT CELL, March 1, 2001; 13(3): 495 - 509. [Abstract] [Full Text]