Genetics, Vol. 149, 479-490, June 1998, Copyright © 1998

Evidence for a Role for AtMYB2 in the Induction of the Arabidopsis Alcohol Dehydrogenase Gene (ADH1) Low Oxygen

Frank U. Hoerena, Rudy Dolferusa,b, Yingru Wua, W. James Peacocka,b, and Elizabeth S. Dennisa,b
a C.S.I.R.O. Plant Industry, Canberra ACT 2601, Australia
b Cooperative Research Centre for Plant Science, Canberra ACT 2601, Australia

Corresponding author: Rudy Dolferus, C.S.I.R.O. Plant Industry, G.P.O. Box 1600, Canberra ACT 2601, Australia, rudy{at}pican.pi.csiro.au (E-mail).

Communicating editor: E. MEYEROWITZ

The transcription factor AtMYB2 binds to two sequence motifs in the promoter of the Arabidopsis ADH1 gene. The binding to the GT-motif (5'-TGGTTT-3') is essential for induction of ADH1 by low oxygen, while binding to the second motif, MBS-2, is not essential for induction. We show that AtMYB2 is induced by hypoxia with kinetics compatible with a role in the regulation of ADH1. Like ADH1, AtMYB2 has root-limited expression. When driven by a constitutive promoter, AtMYB2 is able to transactivate ADH1 expression in transient assays in both Arabidopsis and Nicotiana plumbaginifolia protoplasts, and in particle bombardment of Pisum sativum leaves. Mutation of the GT-motif abolished binding of AtMYB2 and caused loss of activity of the ADH1 promoter in both transient assays and transgenic Arabidopsis plants. These results are consistent with AtMYB2 being a key regulatory factor in the induction of the ADH1 promoter by low oxygen.




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