Genetics, Vol. 149, 45-56, May 1998, Copyright © 1998

Yeast dom34 Mutants Are Defective in Multiple Developmental Pathways and Exhibit Decreased Levels of Polyribosomes

Luther Davisa and JoAnne Engebrechta
a Department of Pharmacological Sciences and Graduate Program in Genetics, State University of New York, Stony Brook, New York 11794

Corresponding author: JoAnne Engebrecht, Department of Pharmacological Sciences, State University of New York, Stony Brook, NY 11794, joanne{at}pharm.sunysb.edu (E-mail).

Communicating editor: F. WINSTON

The DOM34 gene of Saccharomyces cerevisiae is similar to genes found in diverse eukaryotes and archaebacteria. Analysis of dom34 strains shows that progression through the G1 phase of the cell cycle is delayed, mutant cells enter meiosis aberrantly, and their ability to form pseudohyphae is significantly diminished. RPS30A, which encodes ribosomal protein S30, was identified in a screen for high-copy suppressors of the dom34{Delta} growth defect. dom34{Delta} mutants display an altered polyribosome profile that is rescued by expression of RPS30A. Taken together, these data indicate that Dom34p functions in protein translation to promote G1 progression and differentiation. A Drosophila homolog of Dom34p, pelota, is required for the proper coordination of meiosis and spermatogenesis. Heterologous expression of pelota in dom34{Delta} mutants restores wild-type growth and differentiation, suggesting conservation of function between the eukaryotic members of the gene family.




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