Factors Affecting Transposition of the Himar1 mariner Transposon in Vitro

Corresponding author: David J. Lampe, Department of Entomology, University of Illinois at Urbana-Champaign, 505 S. Goodwin, Urbana, IL 61801, d-lampe{at}uiuc.edu (E-mail).

Communicating editor: M. J. SIMMONS

Mariner family transposable elements are widespread in animals, but their regulation is poorly understood, partly because only two are known to be functional. These are particular copies of the Dmmar1 element from Drosophila mauritiana, for example, Mos1, and the consensus sequence of the Himar1 element from the horn fly, Haematobia irritans. An in vitro transposition system was refined to investigate several parameters that influence the transposition of Himar1. Transposition products accumulated linearly over a period of 6 hr. Transposition frequency increased with temperature and was dependent on Mg²⁺ concentration. Transposition frequency peaked over a narrow range of transposase concentration. The decline at higher concentrations, a phenomenon observed in vivo with Mos1, supports the suggestion that mariners may be regulated in part by "overproduction inhibition." Transposition frequency decreased exponentially with increasing transposon size and was affected by the sequence of the flanking DNA of the donor site. A noticeable bias in target site usage suggests a preference for insertion into bent or bendable DNA sequences rather than any specific nucleotide sequences beyond the TA target site.

This article has been cited by other articles:

				K. J. Kines, M. E. Morales, V. H. Mann, G. N. Gobert, and P. J. Brindley Integration of reporter transgenes into Schistosoma mansoni chromosomes mediated by pseudotyped murine leukemia virus FASEB J, August 1, 2008; 22(8): 2936 - 2948. [Abstract] [Full Text] [PDF]

				D. E. Cameron, J. M. Urbach, and J. J. Mekalanos A defined transposon mutant library and its use in identifying motility genes in Vibrio cholerae PNAS, June 24, 2008; 105(25): 8736 - 8741. [Abstract] [Full Text] [PDF]

				C. J. Kristich, V. T. Nguyen, T. Le, A. M. T. Barnes, S. Grindle, and G. M. Dunny Development and Use of an Efficient System for Random mariner Transposon Mutagenesis To Identify Novel Genetic Determinants of Biofilm Formation in the Core Enterococcus faecalis Genome Appl. Envir. Microbiol., June 1, 2008; 74(11): 3377 - 3386. [Abstract] [Full Text] [PDF]

				J. M. Marshall The Impact of Dissociation on Transposon-Mediated Disease Control Strategies Genetics, March 1, 2008; 178(3): 1673 - 1682. [Abstract] [Full Text] [PDF]

				R. A. Subramanian, P. Arensburger, P. W. Atkinson, and D. A. O'Brochta Transposable Element Dynamics of the hAT Element Herves in the Human Malaria Vector Anopheles gambiae s.s. Genetics, August 1, 2007; 176(4): 2477 - 2487. [Abstract] [Full Text] [PDF]

				C. Miskey, B. Papp, L. Mates, L. Sinzelle, H. Keller, Z. Izsvak, and Z. Ivics The Ancient mariner Sails Again: Transposition of the Human Hsmar1 Element by a Reconstructed Transposase and Activities of the SETMAR Protein on Transposon Ends Mol. Cell. Biol., June 15, 2007; 27(12): 4589 - 4600. [Abstract] [Full Text] [PDF]

				A. Urasaki, G. Morvan, and K. Kawakami Functional Dissection of the Tol2 Transposable Element Identified the Minimal cis-Sequence and a Highly Repetitive Sequence in the Subterminal Region Essential for Transposition Genetics, October 1, 2006; 174(2): 639 - 649. [Abstract] [Full Text] [PDF]

				T. M. Maier, R. Pechous, M. Casey, T. C. Zahrt, and D. W. Frank In Vivo Himar1-Based Transposon Mutagenesis of Francisella tularensis. Appl. Envir. Microbiol., March 1, 2006; 72(3): 1878 - 1885. [Abstract] [Full Text] [PDF]

				N. T. Liberati, J. M. Urbach, S. Miyata, D. G. Lee, E. Drenkard, G. Wu, J. Villanueva, T. Wei, and F. M. Ausubel An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants PNAS, February 21, 2006; 103(8): 2833 - 2838. [Abstract] [Full Text] [PDF]

				C. Auge-Gouillou, B. Brillet, M.-H. Hamelin, and Y. Bigot Assembly of the mariner Mos1 Synaptic Complex Mol. Cell. Biol., April 1, 2005; 25(7): 2861 - 2870. [Abstract] [Full Text] [PDF]

				K. Lipkow, N. Buisine, D. J. Lampe, and R. Chalmers Early Intermediates of mariner Transposition: Catalysis without Synapsis of the Transposon Ends Suggests a Novel Architecture of the Synaptic Complex Mol. Cell. Biol., September 15, 2004; 24(18): 8301 - 8311. [Abstract] [Full Text] [PDF]

				E. G. Barry, D. J. Witherspoon, and D. J. Lampe A Bacterial Genetic Screen Identifies Functional Coding Sequences of the Insect mariner Transposable Element Famar1 Amplified From the Genome of the Earwig, Forficula auricularia Genetics, February 1, 2004; 166(2): 823 - 833. [Abstract] [Full Text] [PDF]

				C. Lo, K. Adachi, J. R. Shuster, J. E. Hamer, and L. Hamer The bacterial transposon Tn7 causes premature polyadenylation of mRNA in eukaryotic organisms: TAGKO mutagenesis in filamentous fungi Nucleic Acids Res., August 15, 2003; 31(16): 4822 - 4827. [Abstract] [Full Text] [PDF]

				J. Ashour and M. K. Hondalus Phenotypic Mutants of the Intracellular Actinomycete Rhodococcusequi Created by In Vivo Himar1 Transposon Mutagenesis J. Bacteriol., April 15, 2003; 185(8): 2644 - 2652. [Abstract] [Full Text] [PDF]

				D. J. Lampe, D. J. Witherspoon, F. N. Soto-Adames, and H. M. Robertson Recent Horizontal Transfer of Mellifera Subfamily Mariner Transposons into Insect Lineages Representing Four Different Orders Shows that Selection Acts Only During Horizontal Transfer Mol. Biol. Evol., April 1, 2003; 20(4): 554 - 562. [Abstract] [Full Text] [PDF]

				M. G. Kapetanaki, T. G. Loukeris, I. Livadaras, and C. Savakis High frequencies of Minos transposon mobilization are obtained in insects by using in vitro synthesized mRNA as a source of transposase Nucleic Acids Res., August 1, 2002; 30(15): 3333 - 3340. [Abstract] [Full Text] [PDF]

				C. R. Dietrich, F. Cui, M. L. Packila, J. Li, D. A. Ashlock, B. J. Nikolau, and P. S. Schnable Maize Mu Transposons Are Targeted to the 5' Untranslated Region of the gl8 Gene and Sequences Flanking Mu Target-Site Duplications Exhibit Nonrandom Nucleotide Composition Throughout the Genome Genetics, February 1, 2002; 160(2): 697 - 716. [Abstract] [Full Text] [PDF]

				L. Zhang, A. Dawson, and D. J. Finnegan DNA-binding activity and subunit interaction of the mariner transposase Nucleic Acids Res., September 1, 2001; 29(17): 3566 - 3575. [Abstract] [Full Text] [PDF]

				D. S. Holmes, H.-L. Zhao, G. Levican, J. Ratouchniak, V. Bonnefoy, P. Varela, and E. Jedlicki ISAfe1, an ISL3 Family Insertion Sequence from Acidithiobacillus ferrooxidans ATCC 19859 J. Bacteriol., July 15, 2001; 183(14): 4323 - 4329. [Abstract] [Full Text] [PDF]

				D. J. Lampe, K. K. O. Walden, and H. M. Robertson Loss of Transposase-DNA Interaction May Underlie the Divergence of mariner Family Transposable Elements and the Ability of More than One mariner to Occupy the Same Genome Mol. Biol. Evol., June 1, 2001; 18(6): 954 - 961. [Abstract] [Full Text]

				L. Hamer, K. Adachi, M. V. Montenegro-Chamorro, M. M. Tanzer, S. K. Mahanty, C. Lo, R. W. Tarpey, A. R. Skalchunes, R. W. Heiniger, S. A. Frank, et al. Gene discovery and gene function assignment in filamentous fungi PNAS, April 5, 2001; (2001) 91094198. [Abstract] [Full Text]

				D. L. Hartl Discovery of the Transposable Element Mariner Genetics, February 1, 2001; 157(2): 471 - 476. [Full Text]

				V. Pelicic, S. Morelle, D. Lampe, and X. Nassif Mutagenesis of Neisseria meningitidis by In Vitro Transposition of Himar1 mariner J. Bacteriol., October 1, 2000; 182(19): 5391 - 5398. [Abstract] [Full Text]

				A. M. Gehring, J. R. Nodwell, S. M. Beverley, and R. Losick Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation PNAS, August 6, 2000; (2000) 170059797. [Abstract] [Full Text]

				J. K. Zhang, M. A. Pritchett, D. J. Lampe, H. M. Robertson, and W. W. Metcalf In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1 PNAS, July 30, 2000; (2000) 160272597. [Abstract] [Full Text]

				L. R. O. Tosi and S. M. Beverley cis and trans factors affecting Mos1 mariner evolution and transposition in vitro, and its potential for functional genomics Nucleic Acids Res., February 1, 2000; 28(3): 784 - 790. [Abstract] [Full Text] [PDF]

				T. J. Griffin 4th, L. Parsons, A. E. Leschziner, J. DeVost, K. M. Derbyshire, and N. D. Grindley In vitro transposition of Tn552: a tool for DNA sequencing and mutagenesis Nucleic Acids Res., October 1, 1999; 27(19): 3859 - 3865. [Abstract] [Full Text] [PDF]

				D. J. Lampe, B. J. Akerley, E. J. Rubin, J. J. Mekalanos, and H. M. Robertson Hyperactive transposase mutants of the Himar1 mariner transposon PNAS, September 28, 1999; 96(20): 11428 - 11433. [Abstract] [Full Text] [PDF]

				E. J. Rubin, B. J. Akerley, V. N. Novik, D. J. Lampe, R. N. Husson, and J. J. Mekalanos In vivo transposition of mariner-based elements in enteric bacteria and mycobacteria PNAS, February 16, 1999; 96(4): 1645 - 1650. [Abstract] [Full Text] [PDF]

				L. Hamer, K. Adachi, M. V. Montenegro-Chamorro, M. M. Tanzer, S. K. Mahanty, C. Lo, R. W. Tarpey, A. R. Skalchunes, R. W. Heiniger, S. A. Frank, et al. Gene discovery and gene function assignment in filamentous fungi PNAS, April 24, 2001; 98(9): 5110 - 5115. [Abstract] [Full Text] [PDF]

				J. K. Zhang, M. A. Pritchett, D. J. Lampe, H. M. Robertson, and W. W. Metcalf In vivo transposon mutagenesis of the methanogenic archaeon Methanosarcina acetivorans C2A using a modified version of the insect mariner-family transposable element Himar1 PNAS, August 15, 2000; 97(17): 9665 - 9670. [Abstract] [Full Text] [PDF]

				A. M. Gehring, J. R. Nodwell, S. M. Beverley, and R. Losick Genomewide insertional mutagenesis in Streptomyces coelicolor reveals additional genes involved in morphological differentiation PNAS, August 15, 2000; 97(17): 9642 - 9647. [Abstract] [Full Text] [PDF]