Genetics, Vol. 149, 157-163, May 1998, Copyright © 1998

Mapping of Drosophila Mutations Using Site-Specific Male Recombination

Bin Chena, Tehyen Chua,c, Emily Harmsa,d, J. Peter Gergend,b,c, and Sidney Stricklanda,d,c
a Department of Pharmacology, Institute for Cell and Developmental Biology, University at Stony Brook, Stony Brook, New York 11794-8651
b Department of Biochemistry and Cell Biology, Institute for Cell and Developmental Biology, University at Stony Brook, Stony Brook, New York 11794-8651
c Programs in Molecular and Cellular Biology, Institute for Cell and Developmental Biology, University at Stony Brook, Stony Brook, New York 11794-8651
d Programs in Genetics, Institute for Cell and Developmental Biology, University at Stony Brook, Stony Brook, New York 11794-8651

Corresponding author: Sidney Strickland, Department of Pharmacology, University at Stony Brook, Stony Brook, NY 11794-8651, sid{at}pharm.sunysb.edu (E-mail).

Communicating editor: K. ANDERSON

Although recombination does not usually occur in the male Drosophila germline, site-specific recombination can be induced at the ends of P elements. This finding suggested that male recombination could be used to map Drosophila mutations. In this article, we describe the general method and its application to the mapping of two EMS-induced female-sterile mutations, grauzone and cortex. Within two months, the grauzone gene was mapped relative to seven different P-element insertion sites, and cortex was mapped relative to 23 different P-elements. The results allowed us to map grauzone to a region of about 50 kb, and cortex distal to the chromosomal region 33E. These experiments demonstrate that P-element-induced site-specific male recombination is an efficient and general method to map Drosophila autosomal mutations.




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