Genetics, Vol. 148, 1963-1972, April 1998, Copyright © 1998

Molecular Genetic Dissection of Mouse Unconventional Myosin-VA: Tail Region Mutations

Jian-Dong Huanga, Valerie Mermallb, Marjorie C. Strobela, Liane B. Russelle, Mark S. Moosekerb,c,d, Neal G. Copelanda, and Nancy A. Jenkinsa
a ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, Frederick, Maryland 21702,
b Department of Biology, Yale University, New Haven, Connecticut 06520
c Department of Pathology, Yale University, New Haven, Connecticut 06520
d Department of Cell Biology, Yale University, New Haven, Connecticut 06520
e Oak Ridge National Laboratory, Oak Ridge, Tennessee 37831

Corresponding author: Nancy A. Jenkins, ABL-Basic Research Program, P.O. Box B, Bldg. 539, Frederick, MD 21702-1201, jenkins{at}ncifcrf.gov (E-mail).

Communicating Editor: C. KOZAK

We used an RT-PCR-based sequencing approach to identify the mutations responsible for 17 viable dilute alleles, a mouse-coat-color locus encoding unconventional myosin-VA. Ten of the mutations mapped to the MyoVA tail and are reported here. These mutations represent the first extensive collection of tail mutations reported for any unconventional mammalian myosin. They identify sequences important for tail function and identify domains potentially involved in cargo binding and/or proper folding of the MyoVA tail. Our results also provide support for the notion that different myosin tail isoforms produced by alternative splicing encode important cell-type-specific functions.




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