Cdc1 Is Required for Growth and Mn²⁺ Regulation in Saccharomyces cerevisiae

Corresponding author: Stephen Garrett, Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical Center, 185 South Orange Ave., University Heights, Newark, NJ 07103-2714, garretst{at}umdnj.edu (E-mail).

Communicating editor: M. JOHNSTON

Cdc1 function was initially implicated in bud formation and nuclear division because cdc1(Ts) cells arrested with a small bud, duplicated DNA, and undivided nucleus. Our studies show that Cdc1 is necessary for cell growth at several stages of the cell cycle, as well as in pheromone-treated cells. Thus, Cdc1 depletion might affect bud formation and nuclear division, as well as other cellular processes, by blocking a process involved in general cell growth. Cells depleted of intracellular Mn²⁺ also exhibit a cdc1-like phenotype and recent results suggested Cdc1 might be a Mn²⁺-dependent protein. We show that all of the conditional cdc1(Ts) alleles tested cause cells to become sensitive to Mn²⁺ depletion. In addition, Cdc1 overproduction alleviates the chelator sensitivity of several Mn²⁺ homeostasis mutants. These findings are compatible with a model in which Cdc1 regulates intracellular, and in particular cytosolic, Mn²⁺ levels which, in turn, are necessary for cell growth.

This article has been cited by other articles:

				E. Losev, E. Papanikou, O. W. Rossanese, and B. S. Glick Cdc1p Is an Endoplasmic Reticulum-Localized Putative Lipid Phosphatase That Affects Golgi Inheritance and Actin Polarization by Activating Ca2+ Signaling Mol. Cell. Biol., May 15, 2008; 28(10): 3336 - 3343. [Abstract] [Full Text] [PDF]

				W. Xu, F. J. Smith Jr., R. Subaran, and A. P. Mitchell Multivesicular Body-ESCRT Components Function in pH Response Regulation in Saccharomyces cerevisiae and Candida albicans Mol. Biol. Cell, December 1, 2004; 15(12): 5528 - 5537. [Abstract] [Full Text] [PDF]

				L. Eguez, Y.-S. Chung, A. Kuchibhatla, M. Paidhungat, and S. Garrett Yeast Mn2+ Transporter, Smf1p, Is Regulated by Ubiquitin-Dependent Vacuolar Protein Sorting Genetics, May 1, 2004; 167(1): 107 - 117. [Abstract] [Full Text] [PDF]

				E. E-C. Luk and V. C. Culotta Manganese Superoxide Dismutase in Saccharomyces cerevisiae Acquires Its Metal Co-factor through a Pathway Involving the Nramp Metal Transporter, Smf2p J. Biol. Chem., December 7, 2001; 276(50): 47556 - 47562. [Abstract] [Full Text] [PDF]

				O. W. Rossanese, C. A. Reinke, B. J. Bevis, A. T. Hammond, I. B. Sears, J. O'Connor, and B. S. Glick A Role for Actin, Cdc1p, and Myo2p in the Inheritance of Late Golgi Elements in Saccharomyces cerevisiae J. Cell Biol., March 26, 2001; 153(1): 47 - 62. [Abstract] [Full Text] [PDF]

				K. B. Lengeler, R. C. Davidson, C. D'souza, T. Harashima, W.-C. Shen, P. Wang, X. Pan, M. Waugh, and J. Heitman Signal Transduction Cascades Regulating Fungal Development and Virulence Microbiol. Mol. Biol. Rev., December 1, 2000; 64(4): 746 - 785. [Abstract] [Full Text] [PDF]

				M. Paidhungat and S. Garrett Cdc1 and the Vacuole Coordinately Regulate Mn2+ Homeostasis in the Yeast Saccharomyces cerevisiae Genetics, April 1, 1998; 148(4): 1787 - 1798. [Abstract] [Full Text] [PDF]