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Genetics, Vol. 148, 1777-1786, April 1998, Copyright © 1998

Cdc1 Is Required for Growth and Mn2+ Regulation in Saccharomyces cerevisiae

Madan Paidhungata and Stephen Garrettb
a Department of Pharmacology and Cancer Biology, Duke University Medical Center, Durham, North Carolina 27710
b Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, University Heights, Newark, New Jersey 07103-2714

Corresponding author: Stephen Garrett, Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical Center, 185 South Orange Ave., University Heights, Newark, NJ 07103-2714, garretst{at}umdnj.edu (E-mail).

Communicating editor: M. JOHNSTON

Cdc1 function was initially implicated in bud formation and nuclear division because cdc1(Ts) cells arrested with a small bud, duplicated DNA, and undivided nucleus. Our studies show that Cdc1 is necessary for cell growth at several stages of the cell cycle, as well as in pheromone-treated cells. Thus, Cdc1 depletion might affect bud formation and nuclear division, as well as other cellular processes, by blocking a process involved in general cell growth. Cells depleted of intracellular Mn2+ also exhibit a cdc1-like phenotype and recent results suggested Cdc1 might be a Mn2+-dependent protein. We show that all of the conditional cdc1(Ts) alleles tested cause cells to become sensitive to Mn2+ depletion. In addition, Cdc1 overproduction alleviates the chelator sensitivity of several Mn2+ homeostasis mutants. These findings are compatible with a model in which Cdc1 regulates intracellular, and in particular cytosolic, Mn2+ levels which, in turn, are necessary for cell growth.




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