help button home button Genetics J Clin Inv
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Lee, B.-S.
Right arrow Articles by Garfinkel, D. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lee, B.-S.
Right arrow Articles by Garfinkel, D. J.
Genetics, Vol. 148, 1743-1761, April 1998, Copyright © 1998

Posttranslational Inhibition of Ty1 Retrotransposition by Nucleotide Excision Repair/Transcription Factor TFIIH Subunits Ssl2p and Rad3p

Bum-Soo Leea, Conrad P. Lichtensteina, Brenda Faiolaa, Lori A. Rinckela, William Wysocka, M. Joan Curciob, and David J. Garfinkela
a Gene Regulation and Chromosome Biology Laboratory, Advanced BioScience Laboratories-Basic Research Program, National Cancer Institute-Frederick Cancer Research and Development Center, Frederick, Maryland 21702-1201 and
b Molecular Genetics Program, Wadsworth Center and School of Public Health, State University of New York at Albany, Albany, New York 12201-2002

Corresponding author: David J. Garfinkel, Gene Regulation and Chromosome Biology Laboratory, ABL-Basic Research Program, NCI-Frederick Cancer Research and Development Center, P.O. Box B, Frederick, MD 21702-1201, garfinke{at}ncifcrf.gov (E-mail).

Communicating editor: A. G. HINNEBUSCH

rtt4-1 (r egulator of Ty t ransposition) is a cellular mutation that permits a high level of spontaneous Ty1 retrotransposition in Saccharomyces cerevisiae. The RTT4 gene is allelic with SSL2 (RAD25), which encodes a DNA helicase present in basal transcription (TFIIH) and nucleotide excision repair (NER) complexes. The ssl2-rtt (rtt4-1) mutation stimulates Ty1 retrotransposition, but does not alter Ty1 target site preferences, or increase cDNA or mitotic recombination. In addition to ssl2-rtt, the ssl2-dead and SSL2-1 mutations stimulate Ty1 transposition without altering the level of Ty1 RNA or proteins. However, the level of Ty1 cDNA markedly increases in the ssl2 mutants. Like SSL2, certain mutations in another NER/TFIIH DNA helicase encoded by RAD3 stimulate Ty1 transposition. Although Ssl2p and Rad3p are required for NER, inhibition of Ty1 transposition is independent of Ssl2p and Rad3p NER functions. Our work suggests that NER/TFIIH subunits antagonize Ty1 transposition posttranslationally by inhibiting reverse transcription or destabilizing Ty1 cDNA.




This article has been cited by other articles:


Home page
 GeneticsHome page
K. M. Nyswaner, M. A. Checkley, M. Yi, R. M. Stephens, and D. J. Garfinkel
Chromatin-Associated Genes Protect the Yeast Genome From Ty1 Insertional Mutagenesis
Genetics, January 1, 2008; 178(1): 197 - 214.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. J. Curcio, A. E. Kenny, S. Moore, D. J. Garfinkel, M. Weintraub, E. R. Gamache, and D. T. Scholes
S-Phase Checkpoint Pathways Stimulate the Mobility of the Retrovirus-Like Transposon Ty1
Mol. Cell. Biol., December 15, 2007; 27(24): 8874 - 8885.
[Abstract] [Full Text] [PDF]

Home page
 Eukaryot CellHome page
P. H. Maxwell and M. J. Curcio
Host Factors That Control Long Terminal Repeat Retrotransposons in Saccharomyces cerevisiae: Implications for Regulation of Mammalian Retroviruses
Eukaryot. Cell, July 1, 2007; 6(7): 1069 - 1080.
[Full Text] [PDF]

Home page
 GENES CELLSHome page
F. Mizuki, T. Namiki, H. Sato, H. Furukawa, T. Matsusaka, Y. Ohshima, R. Ishibashi, T. Andoh, and T. Tani
Participation of XPB/Ptr8p, a component of TFIIH, in nucleocytoplasmic transport of mRNA in fission yeast.
Genes Cells, January 1, 2007; 12(1): 35 - 47.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K. Yoder, A. Sarasin, K. Kraemer, M. McIlhatton, F. Bushman, and R. Fishel
The DNA repair genes XPB and XPD defend cells from retroviral infection
PNAS, March 21, 2006; 103(12): 4622 - 4627.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M. E. Gelbart, N. Bachman, J. Delrow, J. D. Boeke, and T. Tsukiyama
Genome-wide identification of Isw2 chromatin-remodeling targets by localization of a catalytically inactive mutant
Genes & Dev., April 15, 2005; 19(8): 942 - 954.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. J. Garfinkel, K. M. Nyswaner, K. M. Stefanisko, C. Chang, and S. P. Moore
Ty1 Copy Number Dynamics in Saccharomyces
Genetics, April 1, 2005; 169(4): 1845 - 1857.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
M. Aye, B. Irwin, N. Beliakova-Bethell, E. Chen, J. Garrus, and S. Sandmeyer
Host Factors That Affect Ty3 Retrotransposition in Saccharomyces cerevisiae
Genetics, November 1, 2004; 168(3): 1159 - 1176.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. J. Radford, M. L. Boyle, C. J. Sheely, J. Graham, D. P. Haeusser, L. Zimmerman, and J. B. Keeney
Increase in Ty1 cDNA Recombination in Yeast sir4 Mutant Strains at High Temperature
Genetics, September 1, 2004; 168(1): 89 - 101.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
N. Bachman, Y. Eby, and J. D. Boeke
Local Definition of Ty1 Target Preference by Long Terminal Repeats and Clustered tRNA Genes
Genome Res., July 1, 2004; 14(7): 1232 - 1247.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
T. Furuchi, T. Takahashi, S. Tanaka, K. Nitta, and A. Naganuma
Functions of yeast helicase Ssl2p that are essential for viability are also involved in protection from the toxicity of adriamycin
Nucleic Acids Res., May 11, 2004; 32(8): 2578 - 2585.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
D. T. Scholes, A. E. Kenny, E. R. Gamache, Z. Mou, and M. J. Curcio
Activation of a LTR-retrotransposon by telomere erosion
PNAS, December 23, 2003; 100(26): 15736 - 15741.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. J. Garfinkel, K. Nyswaner, J. Wang, and J.-Y. Cho
Post-transcriptional Cosuppression of Ty1 Retrotransposition
Genetics, September 1, 2003; 165(1): 83 - 99.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
A. Sundararajan, B.-S. Lee, and D. J. Garfinkel
The Rad27 (Fen-1) Nuclease Inhibits Ty1 Mobility in Saccharomyces cerevisiae
Genetics, January 1, 2003; 163(1): 55 - 67.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. W. Jiang
Transcriptional cosuppression of yeast Ty1 retrotransposons
Genes & Dev., February 15, 2002; 16(4): 467 - 478.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
D. T. Scholes, M. Banerjee, B. Bowen, and M. J. Curcio
Multiple Regulators of Ty1 Transposition in Saccharomyces cerevisiae Have Conserved Roles in Genome Maintenance
Genetics, December 1, 2001; 159(4): 1449 - 1465.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. Bryk, M. Banerjee, D. Conte Jr., and M. J. Curcio
The Sgs1 Helicase of Saccharomyces cerevisiae Inhibits Retrotransposition of Ty1 Multimeric Arrays
Mol. Cell. Biol., August 15, 2001; 21(16): 5374 - 5388.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
M. C. Negritto, J. Qiu, D. O. Ratay, B. Shen, and A. M. Bailis
Novel Function of Rad27 (FEN-1) in Restricting Short-Sequence Recombination
Mol. Cell. Biol., April 1, 2001; 21(7): 2349 - 2358.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
A. Morillon, M. Springer, and P. Lesage
Activation of the Kss1 Invasive-Filamentous Growth Pathway Induces Ty1 Transcription and Retrotransposition in Saccharomyces cerevisiae
Mol. Cell. Biol., August 1, 2000; 20(15): 5766 - 5776.
[Abstract] [Full Text]

Home page
 Mol. Cell. Biol.Home page
B.-S. Lee, L. Bi, D. J. Garfinkel, and A. M. Bailis
Nucleotide Excision Repair/TFIIH Helicases Rad3 and Ssl2 Inhibit Short-Sequence Recombination and Ty1 Retrotransposition by Similar Mechanisms
Mol. Cell. Biol., April 1, 2000; 20(7): 2436 - 2445.
[Abstract] [Full Text]

Home page
 GeneticsHome page
A. J. Rattray, B. K. Shafer, and D. J. Garfinkel
The Saccharomyces cerevisiae DNA Recombination and Repair Functions of the RAD52 Epistasis Group Inhibit Ty1 Transposition
Genetics, February 1, 2000; 154(2): 543 - 556.
[Abstract] [Full Text]

Home page
 GeneticsHome page
H. Huang, J. Y. Hong, C. L. Burck, and S. W. Liebman
Host Genes That Affect the Target-Site Distribution of the Yeast Retrotransposon Ty1
Genetics, April 1, 1999; 151(4): 1393 - 1407.
[Abstract] [Full Text]

Home page
 Mol. Biol. CellHome page
E. Reynaud, H. Lomelí, M. Vázquez, and M. Zurita
The Drosophila melanogaster Homologue of the Xeroderma Pigmentosum D Gene Product Is Located in Euchromatic Regions and Has a Dynamic Response to UV Light-induced Lesions in Polytene Chromosomes
Mol. Biol. Cell, April 1, 1999; 10(4): 1191 - 1203.
[Abstract] [Full Text]

Home page
 GeneticsHome page
S. Maines, M. C. Negritto, X. Wu, G. M. Manthey, and A. M. Bailis
Novel Mutations in the RAD3 and SSL1 Genes Perturb Genome Stability by Stimulating Recombination Between Short Repeats in Saccharomyces cerevisiae
Genetics, November 1, 1998; 150(3): 963 - 976.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1998 by the Genetics Society of America.