- THIS ARTICLE
- Full Text
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by von Borstel, R. C.
- Articles by Steinberg, C. M.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by von Borstel, R. C.
- Articles by Steinberg, C. M.
Topical Reversion at the HIS1 Locus of Saccharomyces cerevisiae 
A Tale of Three Mutants
R. C. von Borstela,
Elizabeth A. Savagea,
Qi Wanga,
Ursula G. G. Henniga,
R. Gary Ritzela,
Grace S.-F. Leea,
Michael D. Hamiltona,
Micah A. Chreneka,
Robert W. Tomaszewskia,
John A. Higginsa,
Christopher J. Tenovea,
Lucia Livierob,
Philip J. Hastingsc,
Christopher T. Korchd, and
C. M. Steinberge
a Department of Biological Sciences, University of Alberta, Edmonton, Alberta T6G 2E9, Canada,
b Istituto di Genetica, Università degli Studi di Parma, I-43100 Parma, Italy,
c Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas 77030,
d P.O. Box 88097, Colorado Springs, Colorado 80908-8097
e Basel Institute for Immunology, CH-4058 Basel, Switzerland
Corresponding author: R. C. von Borstel, Department of Biological Sciences, CW405 Biological Sciences Bldg., University of Alberta, Edmonton, Alberta T6G 2E9, Canada, rc.von-borstel{at}ualberta.ca (E-mail).
Mutants of the HIS1 locus of the yeast Saccharomyces cerevisiae are suitable reporters for spontaneous reversion events because most reversions are topical, that is, within the locus itself. Thirteen mutations of his1-1 now have been identified with respect to base sequence. Revertants of three mutants and their spontaneous reversion rates are presented: (1) a chain termination mutation (his1-208, née his1-1) that does not revert by mutations of tRNA loci and reverts only by intracodonic suppression; (2) a missense mutation (his1-798, née his1-7) that can revert by intragenic suppression by base substitutions of any sort, including a back mutation as well as one three-base deletion; and (3) a -1 frameshift mutation (his1-434, née his1-19) that only reverts topically by +1 back mutation, +1 intragenic suppression, or a -2 deletion. Often the +1 insertion is accompanied by base substitution events at one or both ends of a run of A's. Missense suppressors of his1-798 are either feeders or nonfeeders, and at four different locations within the locus, a single base substitution encoding an amino acid alteration will suffice to turn the nonfeeder phenotype into a feeder phenotype. Late-appearing revertants of his1-798 were found to be slowly growing leaky mutants rather than a manifestation of adaptive mutagenesis. Spontaneous revertants of his1-208 and his1-434 produced no late-arising colonies.
This article has been cited by other articles:
 |
|
 |
|
  N. Erdeniz, S. Dudley, R. Gealy, S. Jinks-Robertson, and R. M. Liskay Novel PMS1 Alleles Preferentially Affect the Repair of Primer Strand Loops during DNA Replication Mol. Cell. Biol., November 1, 2005; 25(21): 9221 - 9231. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. M. Doherty, S. Sharma, L. A. Uzdilla, T. M. Wilson, S. Cui, A. Vindigni, and R. M. Brosh Jr. RECQ1 Helicase Interacts with Human Mismatch Repair Factors That Regulate Genetic Recombination J. Biol. Chem., July 29, 2005; 280(30): 28085 - 28094. [Abstract] [Full Text] [PDF]
|
|