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The Involvement of Cellular Recombination and Repair Genes in RNA-Mediated Recombination in Saccharomyces cerevisiae
Leslie K. Derraa Laboratory of Molecular Microbiology, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20892-0460
Corresponding author: Leslie K. Derr, Laboratory of Molecular Microbiology, National Institutes of Health/National Institute of Allergy and Infectious Diseases, Building 4, Room 311, 4 Center Drive MSC 0460, Bethesda, MD 20892-0460, lderr{at}atlas.niaid.nih.gov (E-mail).
Communicating editor: L. S. SYMINGTON
MscI target sequence, and that this process requires the yeast recombination gene RAD52. In this study, we examine the involvement of other recombination and repair genes in RNA-mediated recombination, and gain insight into the nature of the recombination intermediate. We find that mutation of the mitotic RecA homologs RAD51, RAD55, and RAD57 increases the rate of RNA-mediated recombination relative to the wild type, and that these gene functions are not required for RNA-mediated gene conversion. Interestingly, RAD1 is required for RNA-mediated gene conversion of chromosomal his3-MscI sequences, suggesting that the cDNA intermediate has a region of nonhomology that must be removed during recombination with target sequences. The observation that both RAD1 and RAD52 are required for RNA-mediated gene conversion of chromosomal but not plasmid sequences indicates a clear difference between these two pathways of homologous RNA-mediated recombination.
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