| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Corresponding author: Leslie K. Derr, Laboratory of Molecular Microbiology, National Institutes of Health/National Institute of Allergy and Infectious Diseases, Building 4, Room 311, 4 Center Drive MSC 0460, Bethesda, MD 20892-0460, lderr{at}atlas.niaid.nih.gov (E-mail).
Communicating editor: L. S. SYMINGTON
MscI target sequence, and that this process requires the yeast recombination gene RAD52. In this study, we examine the involvement of other recombination and repair genes in RNA-mediated recombination, and gain insight into the nature of the recombination intermediate. We find that mutation of the mitotic RecA homologs RAD51, RAD55, and RAD57 increases the rate of RNA-mediated recombination relative to the wild type, and that these gene functions are not required for RNA-mediated gene conversion. Interestingly, RAD1 is required for RNA-mediated gene conversion of chromosomal his3-MscI sequences, suggesting that the cDNA intermediate has a region of nonhomology that must be removed during recombination with target sequences. The observation that both RAD1 and RAD52 are required for RNA-mediated gene conversion of chromosomal but not plasmid sequences indicates a clear difference between these two pathways of homologous RNA-mediated recombination.
This article has been cited by other articles:
 |
|
 |
|
  P. H. Maxwell and M. J. Curcio Retrosequence formation restructures the yeast genome Genes & Dev., December 15, 2007; 21(24): 3308 - 3318. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Roy and D. L. Hartl Very little intron loss/gain in Plasmodium: Intron loss/gain mutation rates and intron number Genome Res., June 1, 2006; 16(6): 750 - 756. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. W. Roy and W. Gilbert The pattern of intron loss PNAS, January 18, 2005; 102(3): 713 - 718. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. H. Maxwell, C. Coombes, A. E. Kenny, J. F. Lawler, J. D. Boeke, and M. J. Curcio Ty1 Mobilizes Subtelomeric Y' Elements in Telomerase-Negative Saccharomyces cerevisiae Survivors Mol. Cell. Biol., November 15, 2004; 24(22): 9887 - 9898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Cho, S.-W. Jin, A. Cohen, and R. E. Ellis A Phylogeny of Caenorhabditis Reveals Frequent Loss of Introns During Nematode Evolution Genome Res., July 1, 2004; 14(7): 1207 - 1220. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Mourier and D. C. Jeffares Eukaryotic Intron Loss Science, May 30, 2003; 300(5624): 1393 - 1393. [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Rattray, B. K. Shafer, and D. J. Garfinkel The Saccharomyces cerevisiae DNA Recombination and Repair Functions of the RAD52 Epistasis Group Inhibit Ty1 Transposition Genetics, February 1, 2000; 154(2): 543 - 556. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. GABRIEL and E. H. MULES Fidelity of Retrotransposon Replication Ann. N.Y. Acad. Sci., May 18, 1999; 870(1): 108 - 118. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
