Genetics, Vol. 148, 839-850, February 1998, Copyright © 1998, Genetics Society of America

A Detailed Linkage Map of Rainbow Trout Produced Using Doubled Haploids

William P. Younga, Paul A. Wheelera, Virginia H. Coryellc, Paul Keimc, and Gary H. Thorgaarda,b
a Department of Zoology, Washington State University, Pullman, Washington 99164-4236,
b Department of Genetics and Cell Biology, Washington State University, Pullman, Washington 99164-4234
c Department of Biological Sciences, Northern Arizona University, Flagstaff, Arizona 86011-5640

Corresponding author: Gary H. Thorgaard, Department of Zoology, Washington State University, Pullman, WA 99164-4236, thorglab{at}wsu.edu (E-mail).

Communicating editor: D. BOTSTEIN

We report the first detailed genetic linkage map of rainbow trout (Oncorhynchus mykiss). The segregation analysis was performed using 76 doubled haploid rainbow trout produced by androgenesis from a hybrid between the "OSU" and "Arlee" androgenetically derived homozygous lines. Four hundred and seventy-six markers segregated into 31 major linkage groups and 11 small groups (< 5 markers/group). The minimum genome size is estimated to be 2627.5 cM in length. The sex-determining locus segregated to a distal position on one of the linkage groups. We analyzed the chromosomal distribution of three classes of markers: (1) amplified fragment length polymorphisms, (2) variable number of tandem repeats, and (3) markers obtained using probes homologous to the 5' or 3' end of salmonid-specific small interspersed nuclear elements. Many of the first class of markers were clustered in regions that appear to correspond to centromeres. The second class of markers were more telomeric in distribution, and the third class were intermediate. Tetrasomic inheritance, apparently related to the tetraploid ancestry of salmonid fishes, was detected at one simple sequence repeat locus and suggested by the presence of one extremely large linkage group that appeared to consist of two smaller groups linked at their tips. The double haploid rainbow trout lines and linkage map present a foundation for further genomic studies.




This article has been cited by other articles:


Home page
 GeneticsHome page
K. M. Nichols, A. F. Edo, P. A. Wheeler, and G. H. Thorgaard
The Genetic Basis of Smoltification-Related Traits in Oncorhynchus mykiss
Genetics, July 1, 2008; 179(3): 1559 - 1575.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
K. H Brown, I. R Schultz, and J. J Nagler
Reduced embryonic survival in rainbow trout resulting from paternal exposure to the environmental estrogen 17{alpha}-ethynylestradiol during late sexual maturation
Reproduction, November 1, 2007; 134(5): 659 - 666.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
C. M. Wang, Z. Y. Zhu, L. C. Lo, F. Feng, G. Lin, W. T. Yang, J. Li, and G. H. Yue
A Microsatellite Linkage Map of Barramundi, Lates calcarifer
Genetics, February 1, 2007; 175(2): 907 - 915.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. M. Nichols, K. W. Broman, K. Sundin, J. M. Young, P. A. Wheeler, and G. H. Thorgaard
Quantitative Trait Loci x Maternal Cytoplasmic Environment Interaction for Development Rate in Oncorhynchus mykiss
Genetics, January 1, 2007; 175(1): 335 - 347.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
S. M. Rogers, N. Isabel, and L. Bernatchez
Linkage Maps of the dwarf and Normal Lake Whitefish (Coregonus clupeaformis) Species Complex and Their Hybrids Reveal the Genetic Architecture of Population Divergence
Genetics, January 1, 2007; 175(1): 375 - 398.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
K. Gharbi, A. Gautier, R. G. Danzmann, S. Gharbi, T. Sakamoto, B. Hoyheim, J. B. Taggart, M. Cairney, R. Powell, F. Krieg, et al.
A Linkage Map for Brown Trout (Salmo trutta): Chromosome Homeologies and Comparative Genome Organization With Other Salmonid Fish
Genetics, April 1, 2006; 172(4): 2405 - 2419.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
B.-Y. Lee, W.-J. Lee, J. T. Streelman, K. L. Carleton, A. E. Howe, G. Hulata, A. Slettan, J. E. Stern, Y. Terai, and T. D. Kocher
A Second-Generation Genetic Linkage Map of Tilapia (Oreochromis spp.)
Genetics, May 1, 2005; 170(1): 237 - 244.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
K. S. Reece, W. L. Ribeiro, P. M. Gaffney, R. B. Carnegie, and S. K. Allen Jr
Microsatellite Marker Development and Analysis in the Eastern Oyster (Crassostrea virginica): Confirmation of Null Alleles and Non-Mendelian Segregation Ratios
J. Hered., July 1, 2004; 95(4): 346 - 352.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
T. Moen, K. T. Fjalestad, H. Munck, and L. Gomez-Raya
A Multistage Testing Strategy for Detection of Quantitative Trait Loci Affecting Disease Resistance in Atlantic Salmon
Genetics, June 1, 2004; 167(2): 851 - 858.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
P. B. Samollow, C. M. Kammerer, S. M. Mahaney, J. L. Schneider, S. J. Westenberger, J. L. VandeBerg, and E. S. Robinson
First-Generation Linkage Map of the Gray, Short-Tailed Opossum, Monodelphis domestica, Reveals Genome-Wide Reduction in Female Recombination Rates
Genetics, January 1, 2004; 166(1): 307 - 329.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
K. G. O'Malley, T. Sakamoto, R. G. Danzmann, and M. M. Ferguson
Quantitative Trait Loci for Spawning Date and Body Weight in Rainbow Trout: Testing for Conserved Effects Across Ancestrally Duplicated Chromosomes
J. Hered., July 1, 2003; 94(4): 273 - 284.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
R. A. Woram, K. Gharbi, T. Sakamoto, B. Hoyheim, L.-E. Holm, K. Naish, C. McGowan, M. M. Ferguson, R. B. Phillips, J. Stein, et al.
Comparative Genome Analysis of the Primary Sex-Determining Locus in Salmonid Fishes
Genome Res., February 1, 2003; 13(2): 272 - 280.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. H. J. Kema, S. B. Goodwin, S. Hamza, E. C. P. Verstappen, J. R. Cavaletto, T. A. J. Van der Lee, M. de Weerdt, P. J. M. Bonants, and C. Waalwijk
A Combined Amplified Fragment Length Polymorphism and Randomly Amplified Polymorphism DNA Genetic Linkage Map of Mycosphaerella graminicola, the Septoria Tritici Leaf Blotch Pathogen of Wheat
Genetics, August 1, 2002; 161(4): 1497 - 1505.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
G. C. Waldbieser, B. G. Bosworth, D. J. Nonneman, and W. R. Wolters
A Microsatellite-Based Genetic Linkage Map for Channel Catfish, Ictalurus punctatus
Genetics, June 1, 2001; 158(2): 727 - 734.
[Abstract] [Full Text] [PDF]

Home page
 J HeredHome page
J. L. Martinez, P. Moran, and E. Garcia-Vazquez
A Cryptic RRY(i) Microsatellite From Atlantic Salmon (Salmo salar): Characterization and Chromosomal Location
J. Hered., May 1, 2001; 92(3): 287 - 290.
[Abstract] [Full Text] [PDF]

Home page
 GeneticsHome page
Y.-D. Tan, C. Wan, Y. Zhu, C. Lu, Z. Xiang, and H.-W. Deng
An Amplified Fragment Length Polymorphism Map of the Silkworm
Genetics, March 1, 2001; 157(3): 1277 - 1284.
[Abstract] [Full Text]

Home page
 J HeredHome page
B. D. Robison, P. A. Wheeler, K. Sundin, P. Sikka, and G. H. Thorgaard
Composite Interval Mapping Reveals a Major Locus Influencing Embryonic Development Rate in Rainbow Trout (Oncorhynchus mykiss)
J. Hered., January 1, 2001; 92(1): 16 - 22.
[Abstract] [Full Text] [PDF]

Home page
 Appl. Environ. Microbiol.Home page
L. M. Larraya, G. Pérez, E. Ritter, A. G. Pisabarro, and L. Ramírez
Genetic Linkage Map of the Edible Basidiomycete Pleurotus ostreatus
Appl. Envir. Microbiol., December 1, 2000; 66(12): 5290 - 5300.
[Abstract] [Full Text]

Home page
 Genome ResHome page
I. G. Woods, P. D. Kelly, F. Chu, P. Ngo-Hazelett, Y.-L. Yan, H. Huang, J. H. Postlethwait, and W. S. Talbot
A Comparative Map of the Zebrafish Genome
Genome Res., December 1, 2000; 10(12): 1903 - 1914.
[Abstract] [Full Text]

Home page
 GeneticsHome page
T. Sakamoto, R. G. Danzmann, K. Gharbi, P. Howard, A. Ozaki, S. K. Khoo, R. A. Woram, N. Okamoto, M. M. Ferguson, L.-E. Holm, et al.
A Microsatellite Linkage Map of Rainbow Trout (Oncorhynchus mykiss) Characterized by Large Sex-Specific Differences in Recombination Rates
Genetics, July 1, 2000; 155(3): 1331 - 1345.
[Abstract] [Full Text]

Home page
 Genome ResHome page
P. D. Kelly, F. Chu, I. G. Woods, P. Ngo-Hazelett, T. Cardozo, H. Huang, F. Kimm, L. Liao, Y.-L. Yan, Y. Zhou, et al.
Genetic Linkage Mapping of Zebrafish Genes and ESTs
Genome Res., April 1, 2000; 10(4): 558 - 567.
[Abstract] [Full Text]

Home page
 GeneticsHome page
K. Naruse, S. Fukamachi, H. Mitani, M. Kondo, T. Matsuoka, S. Kondo, N. Hanamura, Y. Morita, K. Hasegawa, R. Nishigaki, et al.
A Detailed Linkage Map of Medaka, Oryzias latipes: Comparative Genomics and Genome Evolution
Genetics, April 1, 2000; 154(4): 1773 - 1784.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
J. D. Hansen and P. Strassburger
Description of an Ectothermic TCR Coreceptor, CD8{alpha}, in Rainbow Trout
J. Immunol., March 15, 2000; 164(6): 3132 - 3139.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
M. Ohtsuka, S. Makino, K. Yoda, H. Wada, K. Naruse, H. Mitani, A. Shima, K. Ozato, M. Kimura, and H. Inoko
Construction of a Linkage Map of the Medaka (Oryzias latipes) and Mapping of the Da Mutant Locus Defective in Dorsoventral Patterning
Genome Res., December 1, 1999; 9(12): 1277 - 1287.
[Abstract] [Full Text]

Home page
 J. Immunol.Home page
J. D. Hansen, P. Strassburger, G. H. Thorgaard, W. P. Young, and L. Du Pasquier
Expression, Linkage, and Polymorphism of MHC-Related Genes in Rainbow Trout, Oncorhynchus mykiss
J. Immunol., July 15, 1999; 163(2): 774 - 786.
[Abstract] [Full Text] [PDF]

Home page
 Genome ResHome page
M. A. Gates, L. Kim, E. S. Egan, T. Cardozo, H. I. Sirotkin, S. T. Dougan, D. Lashkari, R. Abagyan, A. F. Schier, and W. S. Talbot
A Genetic Linkage Map for Zebrafish: Comparative Analysis and Localization of Genes and Expressed Sequences
Genome Res., April 1, 1999; 9(4): 334 - 347.
[Abstract] [Full Text]