Genetic Mapping of Quantitative Trail Loci Affecting Susceptibility to Marek's Disease Virus Induced Tumors in F2 Intercross Chickens

Genetic Mapping of Quantitative Trail Loci Affecting Susceptibility to Marek's Disease Virus Induced Tumors in F₂ Intercross Chickens

Roger L. Vallejo^a, Larry D. Bacon^a, Hsiao-Ching Liu^a, Richard L. Witter^a, Martien A. M. Groenen^b, Jossi Hillel^c, and Hans H. Cheng^a
^a United States Department of Agriculture, Agricultural Research Service, Avian Disease and Oncology Laboratory, East Lansing, Michigan 48823,
^b Department of Animal Breeding, Wageningen Agricultural University, Wageningen, The Netherlands 6700 AH, and
^c Department of Genetics, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel 76100

Corresponding author: Hans H. Cheng, Agricultural Research Service, Avian Disease & Oncology Laboratory, 3606 E. Mount Hope Rd., East Lansing, MI 48823, hcheng{at}pilot.msu.edu (E-mail).

Communicating editor: Z-B. ZENG

Marek's disease (MD) is a lymphoproliferative disease causedby the MD virus (MDV), which costs the poultry industry nearly$1 billion annually. To identify quantitative trait loci (QTL)affecting MD susceptibility, the inbred lines 6₃ (MD resistant)and 7₂ (MD susceptible) were mated to create more than 300 F₂chickens. The F₂ chickens were challenged with MDV (JM strain,moderately virulent) at 1 wk of age and assessed for MD susceptibility.The QTL analysis was divided into three stages. In stage 1,65 DNA markers selected from the chicken genetic maps were typedon the 40 most MD-susceptible and the 40 most MD-resistant F₂chickens, and 21 markers residing near suggestive QTL were revealedby analysis of variance (ANOVA). In stage 2, the suggestivemarkers plus available flanking markers were typed on 272 F₂chickens, and three suggestive QTL were identified by ANOVA.In stage 3, using the interval mapping program Map Manager andpermutation tests, two significant and two suggestive MD QTLwere identified on four chromosomal subregions. Three to fiveloci collected explained between 11 and 23% of the phenotypicMD variation, or 32–68% of the genetic variance. This studyconstitutes the first report in the domestic chicken on themapping of non-major histocompatibility complex QTL affectingMD susceptibility.

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