Regena (Rga), a Drosophila Homolog of the Global Negative Transcriptional Regulator CDC36 (NOT2) from Yeast, Modifies Gene Expression and Suppresses Position Effect Variegation

Regena (Rga), a Drosophila Homolog of the Global Negative Transcriptional Regulator CDC36 (NOT2) from Yeast, Modifies Gene Expression and Suppresses Position Effect Variegation

Maxim V. Frolov^a, Elizaveta V. Benevolenskaya^a, and James A. Birchler^a
^a Division of Biological Sciences, University of Missouri–Columbia, Columbia, Missouri 65211

Corresponding author: James A. Birchler, 117 Tucker Hall, University of Missouri-Columbia, Columbia MO 65211, birchler{at}biosci.mbp.missouri.edu (E-mail).

Communicating editor: R. S. HAWLEY

A mutation in Regena (Rga) was isolated in screens for modifiersof white eye color gene expression. The reduction in the levelof the Rga product results in a complex modulation of whitemRNA both positively and negatively, depending on the developmentalstage. In addition to white, Rga also affects the expressionof several other tested genes, with one of them, Vinculin, beingregulated in a strong sex-specific manner. Rga was cloned bytransposon tagging. Its predicted product lacks any recognizednucleic acid–binding motif but is homologous to a globalnegative transcriptional regulator, CDC36 (NOT2), from yeast.Rga also acts as a suppressor of position effect variegation,suggesting that a possible function of Rga could be mediationof an interaction between chromatin proteins and the transcriptionalcomplex.

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