Genetics, Vol. 148, 251-266, January 1998, Copyright © 1998, Genetics Society of America

Genetic Analysis of brahma: The Drosophila Homolog of the Yeast Chromatin Remodeling Factor SWI2/SNF2

Lisa K. Elfringa, Carla Daniela, Ophelia Papoulasa, Renate Deuringa, Melinda Sartea, Sarah Moseleya, Shelley J. Beeka, W. Ross Waldripa, Gary Daubressea, Angela DePacea, James A. Kennisonb, and John W. Tamkuna
a Department of Biology, University of California, Santa Cruz, California 95064,
b Laboratory of Molecular Genetics, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892

Corresponding author: John W. Tamkun, 350 Sinsheimer Labs, Department of Biology, University of California, Santa Cruz, CA 95064, tamkun{at}biology.ucsc.edu (E-mail).

Communicating editor: S. HENIKOFF

The Drosophila brahma (brm) gene encodes an activator of homeotic genes related to the yeast chromatin remodeling factor SWI2/SNF2. Here, we report the phenotype of null and dominant-negative brm mutations. Using mosaic analysis, we found that the complete loss of brm function decreases cell viability and causes defects in the peripheral nervous system of the adult. A dominant-negative brm mutation was generated by replacing a conserved lysine in the ATP-binding site of the BRM protein with an arginine. This mutation eliminates brm function in vivo but does not affect assembly of the 2-MD BRM complex. Expression of the dominant-negative BRM protein caused peripheral nervous system defects, homeotic transformations, and decreased viability. Consistent with these findings, the BRM protein is expressed at relatively high levels in nuclei throughout the developing organism. Site-directed mutagenesis was used to investigate the functions of conserved regions of the BRM protein. Domain II is essential for brm function and is required for the assembly or stability of the BRM complex. In spite of its conservation in numerous eukaryotic regulatory proteins, the deletion of the bromodomain of the BRM protein has no discernible phenotype.




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