- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Terauchi, R.
- Articles by Miyashita, N. T.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Terauchi, R.
- Articles by Miyashita, N. T.
Genetics, Vol 147, 1899-1914, Copyright © 1997
INVESTIGATIONS |
DNA Polymorphism at the Pgi Locus of a Wild Yam, Dioscorea tokoro
R. Terauchi, T. Terachi and N. T. Miyashita
Laboratory of Plant Systematics, Department of Botany, Kyoto University, Kyoto 606-01, Japan, Plant Molecular Biology Group, Biocenter, University of Frankfurt, Frankfurt am Main D-60439, Germany
To study the origin and maintenance mechanisms of the PGI allozyme polymorphism of a wild plant, Dioscorea tokoro, DNA sequences of the entire coding region (1701 bp) and two intronic regions (total 2049 bp) of the Pgi gene as well as a part of the Adh gene (590 bp) were analyzed. Two replacement substitutions were revealed to be responsible for the differentiation of three allozymes alleles (Pgi-a, Pgi-b and Pgi-c) that occur in natural population in intermediate frequencies. Interspecific comparison of DNA sequences identified Pgi-b as the oldest allele, from which two other alleles were derived probably within the last 150,000 years. The level of DNA polymorphism at D. tokoro Pgi locus was low. No elevated level of DNA polymorphism was detected in the close vicinity of the two replacement sites differentiating the three allozymes. Departures from the neutral mutation hypothesis were detected by Fu and Li's and MK tests. The observed patterns of DNA polymorphism are explainable by both (1) the neutral mutation hypothesis with an assumption of small effective size of D. tokoro population, and (2) the positive selection hypothesis that the allele frequencies of Pgi-a and Pgi-c have increased in a short time by their selective advantages.
This article has been cited by other articles:
 |
|
 |
|
  N. E. Rank, D. A. Bruce, D. M. McMillan, C. Barclay, and E. P. Dahlhoff Phosphoglucose isomerase genotype affects running speed and heat shock protein expression after exposure to extreme temperatures in a montane willow beetle J. Exp. Biol., March 1, 2007; 210(5): 750 - 764. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. S Ford, J. Lee, B. G Baldwin, and L. D Gottlieb Species divergence and relationships in Stephanomeria (Compositae): PgiC phylogeny compared to prior biosystematic studies Am. J. Botany, March 1, 2006; 93(3): 480 - 490. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y.-C. Chiang, B. A. Schaal, C.-H. Chou, S. Huang, and T.-Y. Chiang Contrasting selection modes at the Adh1 locus in outcrossing Miscanthus sinensis vs. inbreeding Miscanthus condensatus (Poaceae) Am. J. Botany, April 1, 2003; 90(4): 561 - 570. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. D. Gottlieb and V. S. Ford The 5' Leader of Plant PgiC has an Intron: The Leader Shows Both the Loss and Maintenance of Constraints Compared with Introns and Exons in the Coding Region Mol. Biol. Evol., September 1, 2002; 19(9): 1613 - 1623. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. Dvornyk, A. Sirvio, M. Mikkonen, and O. Savolainen Low Nucleotide Diversity at the pal1 Locus in the Widely Distributed Pinus sylvestris Mol. Biol. Evol., February 1, 2002; 19(2): 179 - 188. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. C. Verrelli and W. F. Eanes Clinal Variation for Amino Acid Polymorphisms at the Pgm Locus in Drosophila melanogaster Genetics, April 1, 2001; 157(4): 1649 - 1663. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kawabe, K. Yamane, and N. T. Miyashita DNA Polymorphism at the Cytosolic Phosphoglucose Isomerase (PgiC) Locus of the Wild Plant Arabidopsis thaliana Genetics, November 1, 2000; 156(3): 1339 - 1347. [Abstract] [Full Text]
|
|
|
|
|
|
D. A. Filatov and D. Charlesworth DNA Polymorphism, Haplotype Structure and Balancing Selection in the Leavenworthia PgiC Locus Genetics, November 1, 1999; 153(3): 1423 - 1434. [Abstract] [Full Text]
|
|
|
|
|
|
A. Kawabe and N. T. Miyashita DNA Variation in the Basic Chitinase Locus (ChiB) Region of the Wild Plant Arabidopsis thaliana Genetics, November 1, 1999; 153(3): 1445 - 1453. [Abstract] [Full Text]
|
|
|
|
|
|
A. Navarro-Sabate, M. Aguade, and C. Segarra The Relationship Between Allozyme and Chromosomal Polymorphism Inferred From Nucleotide Variation at the Acph-1 Gene Region of Drosophila subobscura Genetics, October 1, 1999; 153(2): 871 - 889. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Innan, R. Terauchi, G. Kahl, and F. Tajima A Method for Estimating Nucleotide Diversity From AFLP Data Genetics, March 1, 1999; 151(3): 1157 - 1164. [Abstract] [Full Text]
|
|
|
|
|
|
F. Liu, D. Charlesworth, and M. Kreitman The Effect of Mating System Differences on Nucleotide Diversity at the Phosphoglucose Isomerase Locus in the Plant Genus Leavenworthia Genetics, January 1, 1999; 151(1): 343 - 357. [Abstract] [Full Text]
|
|
|
|
 |
|
 M. P. Cummings and M. T. Clegg Nucleotide sequence diversity at the alcohol dehydrogenase 1 locus in wild barley (Hordeum vulgare ssp. spontaneum): An evaluation of the background selection hypothesis PNAS, May 12, 1998; 95(10): 5637 - 5642. [Abstract] [Full Text] [PDF]
|
|