Genetics, Vol 147, 1697-1722, Copyright © 1997

INVESTIGATIONS

P-Element Insertion Alleles of Essential Genes on the Third Chromosome of Drosophila melanogaster: Correlation of Physical and Cytogenetic Maps in Chromosomal Region 86E-87F

P. Deak, M. M. Omar, RDC. Saunders, M. Pal, O. Komonyi, J. Szidonya, P. Maroy, Y. Zhang, M. Ashburner, P. Benos, C. Savakis, I. Siden-Kiamos, C. Louis, V. N. Bolshakov, F. C. Kafatos, E. Madueno, J. Modolell and D. M. Glover
Department of Anatomy and Physiology, University of Dundee, Dundee DD1 4HN, United Kingdom, Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, H-6701 Szeged, Hungary

We have established a collection of 2460 lethal or semi-lethal mutant lines using a procedure thought to insert single P elements into vital genes on the third chromosome of Drosophila melanogaster. More than 1200 randomly selected lines were examined by in situ hybridization and 90% found to contain single insertions at sites that mark 89% of all lettered subdivisions of the Bridges' map. A set of chromosomal deficiencies that collectively uncover ~25% of the euchromatin of chromosome 3 reveal lethal mutations in 468 lines corresponding to 145 complementation groups. We undertook a detailed analysis of the cytogenetic interval 86E-87F and identified 87 P-element-induced mutations falling into 38 complementation groups, 16 of which correspond to previously known genes. Twenty-one of these 38 complementation groups have at least one allele that has a P-element insertion at a position consistent with the cytogenetics of the locus. We have rescued P elements and flanking chromosomal sequences from the 86E-87F region in 35 lines with either lethal or genetically silent P insertions, and used these as probes to identify cosmids and P1 clones from the Drosophila genome projects. This has tied together the physical and genetic maps and has linked 44 previously identified cosmid contigs into seven ``supercontigs'' that span the interval. STS data for sequences flanking one side of the P-element insertions in 49 lines has identified insertions in the {alpha}{gamma} element at 87C, two known transposable elements, and the open reading frames of seven putative single copy genes. These correspond to five known genes in this interval, and two genes identified by the homology of their predicted products to known proteins from other organisms.

This article has been cited by other articles:

				H. Duan, C. Zhang, J. Chen, H. Sink, E. Frei, and M. Noll A key role of Pox meso in somatic myogenesis of Drosophila Development, November 15, 2007; 134(22): 3985 - 3997. [Abstract] [Full Text] [PDF]

				B. Williams, G. Leung, H. Maiato, A. Wong, Z. Li, E. V. Williams, C. Kirkpatrick, C. F. Aquadro, C. L. Rieder, and M. L. Goldberg Mitch a rapidly evolving component of the Ndc80 kinetochore complex required for correct chromosome segregation in Drosophila J. Cell Sci., October 15, 2007; 120(20): 3522 - 3533. [Abstract] [Full Text] [PDF]

				J. Rohrbough, E. Rushton, E. Woodruff III, T. Fergestad, K. Vigneswaran, and K. Broadie Presynaptic establishment of the synaptic cleft extracellular matrix is required for post-synaptic differentiation Genes & Dev., October 15, 2007; 21(20): 2607 - 2628. [Abstract] [Full Text] [PDF]

				Y.-C. Kim, H.-G. Lee, and K.-A. Han D1 Dopamine Receptor dDA1 Is Required in the Mushroom Body Neurons for Aversive and Appetitive Learning in Drosophila J. Neurosci., July 18, 2007; 27(29): 7640 - 7647. [Abstract] [Full Text] [PDF]

				R. McCaffrey, D. St Johnston, and A. Gonzalez-Reyes Drosophila mus301/spindle-C Encodes a Helicase With an Essential Role in Double-Strand DNA Break Repair and Meiotic Progression Genetics, November 1, 2006; 174(3): 1273 - 1285. [Abstract] [Full Text] [PDF]

				M. K. Gatt and D. M. Glover The Drosophila phosphatidylinositol transfer protein encoded by vibrator is essential to maintain cleavage-furrow ingression in cytokinesis J. Cell Sci., June 1, 2006; 119(11): 2225 - 2235. [Abstract] [Full Text] [PDF]

				U. Abdu, D. Bar, and T. Schupbach spn-F encodes a novel protein that affects oocyte patterning and bristle morphology in Drosophila Development, April 15, 2006; 133(8): 1477 - 1484. [Abstract] [Full Text] [PDF]

				F. Marcillac, F. Bousquet, J. Alabouvette, F. Savarit, and J.-F. Ferveur A Mutation With Major Effects on Drosophila melanogaster Sex Pheromones Genetics, December 1, 2005; 171(4): 1617 - 1628. [Abstract] [Full Text] [PDF]

				R. D. Read, P. J. Goodfellow, E. R. Mardis, N. Novak, J. R. Armstrong, and R. L. Cagan A Drosophila Model of Multiple Endocrine Neoplasia Type 2 Genetics, November 1, 2005; 171(3): 1057 - 1081. [Abstract] [Full Text] [PDF]

				R. B. Beckstead, S. S. Ner, K. G. Hales, T. A. Grigliatti, B. S. Baker, and H. J. Bellen Bonus, a Drosophila TIF1 Homolog, Is a Chromatin-Associated Protein That Acts as a Modifier of Position-Effect Variegation Genetics, February 1, 2005; 169(2): 783 - 794. [Abstract] [Full Text] [PDF]

				M. Angulo, M. Corominas, and F. Serras Activation and repression activities of ash2 in Drosophila wing imaginal discs Development, October 15, 2004; 131(20): 4943 - 4953. [Abstract] [Full Text] [PDF]

				A. Lundstrom, M. Gallio, C. Englund, P. Steneberg, J. Hemphala, P. Aspenstrom, K. Keleman, L. Falileeva, B. J. Dickson, and C. Samakovlis Vilse, a conserved Rac/Cdc42 GAP mediating Robo repulsion in tracheal cells and axons Genes & Dev., September 1, 2004; 18(17): 2161 - 2171. [Abstract] [Full Text] [PDF]

				R. D. Read, E. A. Bach, and R. L. Cagan Drosophila C-Terminal Src Kinase Negatively Regulates Organ Growth and Cell Proliferation through Inhibition of the Src, Jun N-Terminal Kinase, and STAT Pathways Mol. Cell. Biol., August 1, 2004; 24(15): 6676 - 6689. [Abstract] [Full Text] [PDF]

				M. Gallio, C. Englund, P. Kylsten, and C. Samakovlis Rhomboid 3 orchestrates Slit-independent repulsion of tracheal branches at the CNS midline Development, August 1, 2004; 131(15): 3605 - 3614. [Abstract] [Full Text] [PDF]

				M. S. Savoian, M. K. Gatt, M. G. Riparbelli, G. Callaini, and D. M. Glover Drosophila Klp67A is required for proper chromosome congression and segregation during meiosis I J. Cell Sci., July 15, 2004; 117(16): 3669 - 3677. [Abstract] [Full Text] [PDF]

				P. P. D'Avino, M. S. Savoian, and D. M. Glover Mutations in sticky lead to defective organization of the contractile ring during cytokinesis and are enhanced by Rho and suppressed by Rac J. Cell Biol., July 5, 2004; 166(1): 61 - 71. [Abstract] [Full Text] [PDF]

				H. J. Bellen, R. W. Levis, G. Liao, Y. He, J. W. Carlson, G. Tsang, M. Evans-Holm, P. R. Hiesinger, K. L. Schulze, G. M. Rubin, et al. The BDGP Gene Disruption Project: Single Transposon Insertions Associated With 40% of Drosophila Genes Genetics, June 1, 2004; 167(2): 761 - 781. [Abstract] [Full Text] [PDF]

				R. Gandhi, S. Bonaccorsi, D. Wentworth, S. Doxsey, M. Gatti, and A. Pereira The Drosophila Kinesin-like Protein KLP67A Is Essential for Mitotic and Male Meiotic Spindle Assembly Mol. Biol. Cell, January 1, 2004; 15(1): 121 - 131. [Abstract] [Full Text] [PDF]

				C. C. Milton, B. Huynh, P. Batterham, S. L. Rutherford, and A. A. Hoffmann Quantitative trait symmetry independent of Hsp90 buffering: Distinct modes of genetic canalization and developmental stability PNAS, November 11, 2003; 100(23): 13396 - 13401. [Abstract] [Full Text] [PDF]

				S. Beltran, E. Blanco, F. Serras, B. Perez-Villamil, R. Guigo, S. Artavanis-Tsakonas, and M. Corominas Transcriptional network controlled by the trithorax-group gene ash2 in Drosophila melanogaster PNAS, March 18, 2003; 100(6): 3293 - 3298. [Abstract] [Full Text] [PDF]

				H. Liu, M. Mardahl-Dumesnil, S. T. Sweeney, C. J. O'Kane, and S. I. Bernstein Drosophila paramyosin is important for myoblast fusion and essential for myofibril formation J. Cell Biol., March 17, 2003; 160(6): 899 - 908. [Abstract] [Full Text] [PDF]

				T. Szlanka, L. Haracska, I. Kiss, P. Deak, E. Kurucz, I. Ando, E. Viragh, and A. Udvardy Deletion of proteasomal subunit S5a/Rpn10/p54 causes lethality, multiple mitotic defects and overexpression of proteasomal genes in Drosophila melanogaster J. Cell Sci., March 15, 2003; 116(6): 1023 - 1033. [Abstract] [Full Text] [PDF]

				J. Hemphala, A. Uv, R. Cantera, S. Bray, and C. Samakovlis Grainy head controls apical membrane growth and tube elongation in response to Branchless/FGF signalling Development, March 2, 2003; 130(2): 249 - 258. [Abstract] [Full Text] [PDF]

				E. Mathe, Y. H. Inoue, W. Palframan, G. Brown, and D. M. Glover Orbit/Mast, the CLASP orthologue of Drosophila, is required for asymmetric stem cell and cystocyte divisions and development of the polarised microtubule network that interconnects oocyte and nurse cells during oogenesis Development, March 1, 2003; 130(5): 901 - 915. [Abstract] [Full Text] [PDF]

				D. C. Presgraves A Fine-Scale Genetic Analysis of Hybrid Incompatibilities in Drosophila Genetics, March 1, 2003; 163(3): 955 - 972. [Abstract] [Full Text] [PDF]

				G. Mirey, M. Balakireva, S. L'Hoste, C. Rosse, S. Voegeling, and J. Camonis A Ral Guanine Exchange Factor-Ral Pathway Is Conserved in Drosophila melanogaster and Sheds New Light on the Connectivity of the Ral, Ras, and Rap Pathways Mol. Cell. Biol., February 1, 2003; 23(3): 1112 - 1124. [Abstract] [Full Text]

				K. Nairz, H. Stocker, B. Schindelholz, and E. Hafen High-resolution SNP mapping by denaturing HPLC PNAS, August 6, 2002; 99(16): 10575 - 10580. [Abstract] [Full Text] [PDF]

				K. P. Sem, B. Zahedi, I. Tan, M. Deak, L. Lim, and N. Harden ACK Family Tyrosine Kinase Activity Is a Component of Dcdc42 Signaling during Dorsal Closure in Drosophila melanogaster Mol. Cell. Biol., June 1, 2002; 22(11): 3685 - 3697. [Abstract] [Full Text] [PDF]

				N. Harden, M. Ricos, K. Yee, J. Sanny, C. Langmann, H. Yu, W. Chia, and L. Lim Drac1 and Crumbs participate in amnioserosa morphogenesis during dorsal closure in Drosophila J. Cell Sci., May 15, 2002; 115(10): 2119 - 2129. [Abstract] [Full Text] [PDF]

				P. E. D. Lachance, M. Miron, B. Raught, N. Sonenberg, and P. Lasko Phosphorylation of Eukaryotic Translation Initiation Factor 4E Is Critical for Growth Mol. Cell. Biol., March 15, 2002; 22(6): 1656 - 1663. [Abstract] [Full Text] [PDF]

				R. Dubruille, A. Laurencon, C. Vandaele, E. Shishido, M. Coulon-Bublex, P. Swoboda, P. Couble, M. Kernan, and B. Durand Drosophila Regulatory factor X is necessary for ciliated sensory neuron differentiation Development, January 12, 2002; 129(23): 5487 - 5498. [Abstract] [Full Text] [PDF]

				H. L.-S. a. D. St. Johnston Delta signaling from the germ line controls the proliferation and differentiation of the somatic follicle cells during Drosophila oogenesis Genes & Dev., June 1, 2001; 15(11): 1393 - 1405. [Abstract] [Full Text] [PDF]

				P. V. Benos, M. K. Gatt, L. Murphy, D. Harris, B. Barrell, C. Ferraz, S. Vidal, C. Brun, J. Demaille, E. Cadieu, et al. From First Base: The Sequence of the Tip of the X Chromosome of Drosophila melanogaster, a Comparison of Two Sequencing Strategies Genome Res., May 1, 2001; 11(5): 710 - 730. [Abstract] [Full Text]

				D. Maier, C. L. Farr, B. Poeck, A. Alahari, M. Vogel, S. Fischer, L. S. Kaguni, and S. Schneuwly Mitochondrial Single-stranded DNA-binding Protein Is Required for Mitochondrial DNA Replication and Development in Drosophila melanogaster Mol. Biol. Cell, April 1, 2001; 12(4): 821 - 830. [Abstract] [Full Text]

				S. Kuhfittig, J. Szabad, G. Schotta, J. Hoffmann, E. Máthé, and G. Reuter pitkinD, a Novel Gain-of-Function Enhancer of Position-Effect Variegation, Affects Chromatin Regulation During Oogenesis and Early Embryogenesis in Drosophila Genetics, March 1, 2001; 157(3): 1227 - 1244. [Abstract] [Full Text]

				E. Kurant, D. Eytan, and A. Salzberg Mutational Analysis of the Drosophila homothorax Gene Genetics, February 1, 2001; 157(2): 689 - 698. [Abstract] [Full Text]

				I Monge, R Krishnamurthy, D Sims, F Hirth, M Spengler, L Kammermeier, H Reichert, and P. Mitchell Drosophila transcription factor AP-2 in proboscis, leg and brain central complex development Development, January 4, 2001; 128(8): 1239 - 1252. [Abstract] [PDF]

				W. Geng, B. He, M. Wang, and P. N. Adler The tricornered Gene, Which Is Required for the Integrity of Epidermal Cell Extensions, Encodes the Drosophila Nuclear DBF2-Related Kinase Genetics, December 1, 2000; 156(4): 1817 - 1828. [Abstract] [Full Text]

				G. Roman, J. He, and R. L. Davis kurtz, a Novel Nonvisual Arrestin, Is an Essential Neural Gene in Drosophila Genetics, July 1, 2000; 155(3): 1281 - 1295. [Abstract] [Full Text]

				M. Balakireva, N. Gendre, R. F. Stocker, and J.-F. Ferveur The Genetic Variant Voila1 Causes Gustatory Defects during Drosophila Development J. Neurosci., May 1, 2000; 20(9): 3425 - 3433. [Abstract] [Full Text] [PDF]

				Y. H. Inoue, M. do Carmo Avides, M. Shiraki, P. Deak, M. Yamaguchi, Y. Nishimoto, A. Matsukage, and D. M. Glover Orbit, a Novel Microtubule-associated Protein Essential for Mitosis in Drosophila melanogaster J. Cell Biol., April 3, 2000; 149(1): 153 - 166. [Abstract] [Full Text] [PDF]

				Y Zhang, D Featherstone, W Davis, E Rushton, and K Broadie Drosophila D-titin is required for myoblast fusion and skeletal muscle striation J. Cell Sci., January 9, 2000; 113(17): 3103 - 3115. [Abstract] [PDF]

				J. Brill, G. Hime, M Scharer-Schuksz, and M. Fuller A phospholipid kinase regulates actin organization and intercellular bridge formation during germline cytokinesis Development, January 9, 2000; 127(17): 3855 - 3864. [Abstract] [PDF]

				S. Martini, G Roman, S Meuser, G Mardon, and R. Davis The retinal determination gene, dachshund, is required for mushroom body cell differentiation Development, January 6, 2000; 127(12): 2663 - 2672. [Abstract] [PDF]

				A. Cadavid, A Ginzel, and J. Fischer The function of the Drosophila fat facets deubiquitinating enzyme in limiting photoreceptor cell number is intimately associated with endocytosis Development, January 4, 2000; 127(8): 1727 - 1736. [Abstract] [PDF]

				M. Y. Yang, Z. Wang, M. MacPherson, J. A. T. Dow, and K. Kaiser A Novel Drosophila Alkaline Phosphatase Specific to the Ellipsoid Body of the Adult Brain and the Lower Malpighian (Renal) Tubule Genetics, January 1, 2000; 154(1): 285 - 297. [Abstract] [Full Text]

				C. F. Cullen, P. Deak, D. M. Glover, and H. Ohkura mini spindles: A Gene Encoding a Conserved Microtubule-associated Protein Required for the Integrity of the Mitotic Spindle in Drosophila J. Cell Biol., September 6, 1999; 146(5): 1005 - 1018. [Abstract] [Full Text] [PDF]

				A. C. Spradling, D. Stern, A. Beaton, E. J. Rhem, T. Laverty, N. Mozden, S. Misra, and G. M. Rubin The Berkeley Drosophila Genome Project Gene Disruption Project: Single P-Element Insertions Mutating 25% of Vital Drosophila Genes Genetics, September 1, 1999; 153(1): 135 - 177. [Abstract] [Full Text]

				G. Toba, T. Ohsako, N. Miyata, T. Ohtsuka, K.-H. Seong, and T. Aigaki The Gene Search System: A Method for Efficient Detection and Rapid Molecular Identification of Genes in Drosophila melanogaster Genetics, February 1, 1999; 151(2): 725 - 737. [Abstract] [Full Text]

				O Zilian, E Frei, R Burke, D Brentrup, T Gutjahr, P. Bryant, and M Noll double-time is identical to discs overgrown, which is required for cell survival, proliferation and growth arrest in Drosophila imaginal discs Development, January 12, 1999; 126(23): 5409 - 5420. [Abstract] [PDF]

				Z. Chamoun, R. K. Mann, D. Nellen, D. P. von Kessler, M. Bellotto, P. A. Beachy, and K. Basler Skinny Hedgehog, an Acyltransferase Required for Palmitoylation and Activity of the Hedgehog Signal Science, September 14, 2001; 293(5537): 2080 - 2084. [Abstract] [Full Text] [PDF]

				M.-L. Parmentier, D. Woods, S. Greig, P. G. Phan, A. Radovic, P. Bryant, and C. J. O'Kane Rapsynoid/Partner of Inscuteable Controls Asymmetric Division of Larval Neuroblasts in Drosophila J. Neurosci., July 15, 2000; 20(14): RC84 - RC84. [Abstract] [Full Text] [PDF]

				F. J.M. van Eeden, I. M. Palacios, M. Petronczki, M. J.D. Weston, and D. St Johnston Barentsz is essential for the posterior localization of oskar mRNA and colocalizes with it to the posterior pole J. Cell Biol., August 6, 2001; 154(3): 511 - 524. [Abstract] [Full Text] [PDF]