Genetics, Vol 147, 1557-1568, Copyright © 1997

INVESTIGATIONS

The Saccharomyces cerevisiae RAD30 Gene, a Homologue of Escherichia coli dinB and umuC, Is DNA Damage Inducible and Functions in a Novel Error-Free Postreplication Repair Mechanism

J. P. McDonald, A. S. Levine and R. Woodgate
Section on DNA Replication, Repair and Mutagenesis, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892-2725

Damage-inducible mutagenesis in prokaryotes is largely dependent upon the activity of the UmuD'C-like proteins. Since many DNA repair processes are structurally and/or functionally conserved between prokaryotes and eukaryotes, we investigated the role of RAD30, a previously uncharacterized Saccharomyces cerevisiae DNA repair gene related to the Escherichia coli dinB, umuC and S. cerevisiae REV1 genes, in UV resistance and UV-induced mutagenesis. Similar to its prokaryotic homologues, RAD30 was found to be damage inducible. Like many S. cerevisiae genes involved in error-prone DNA repair, epistasis analysis clearly places RAD30 in the RAD6 group and rad30 mutants display moderate UV sensitivity reminiscent of rev mutants. However, unlike rev mutants, no defect in UV-induced reversion was seen in rad30 strains. While rad6 and rad18 are both epistatic to rad30, no epistasis was observed with rev1, rev3, rev7 or rad5, all of which are members of the RAD6 epistasis group. These findings suggest that RAD30 participates in a novel error-free repair pathway dependent on RAD6 and RAD18, but independent of REV1, REV3, REV7 and RAD5.


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A. Yamada, C. Masutani, S. Iwai, and F. Hanaoka
Complementation of defective translesion synthesis and UV light sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase {eta}
Nucleic Acids Res., July 1, 2000; 28(13): 2473 - 2480.
[Abstract] [Full Text] [PDF]

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 Genes Dev.Home page
A. Tissier, J. P. McDonald, E. G. Frank, and R. Woodgate
poliota , a remarkably error-prone human DNA polymerase
Genes & Dev., July 1, 2000; 14(13): 1642 - 1650.
[Abstract] [Full Text]

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 Mol. Cell. Biol.Home page
K. Tanaka, T. Yonekawa, Y. Kawasaki, M. Kai, K. Furuya, M. Iwasaki, H. Murakami, M. Yanagida, and H. Okayama
Fission Yeast Eso1p Is Required for Establishing Sister Chromatid Cohesion during S Phase
Mol. Cell. Biol., May 15, 2000; 20(10): 3459 - 3469.
[Abstract] [Full Text]

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 Proc. Natl. Acad. Sci. USAHome page
R. E. Johnson, S. Prakash, and L. Prakash
The human DINB1 gene encodes the DNA polymerase Poltheta
PNAS, April 11, 2000; 97(8): 3838 - 3843.
[Abstract] [Full Text] [PDF]

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 Proc. Natl. Acad. Sci. USAHome page
P. E. M. Gibbs, X.-D. Wang, Z. Li, T. P. McManus, W. G. McGregor, C. W. Lawrence, and V. M. Maher
The function of the human homolog of Saccharomyces cerevisiae REV1 is required for mutagenesis induced by UV light
PNAS, April 11, 2000; 97(8): 4186 - 4191.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
R. E. Johnson, M. T. Washington, S. Prakash, and L. Prakash
Fidelity of Human DNA Polymerase eta
J. Biol. Chem., March 10, 2000; 275(11): 7447 - 7450.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
F. Yuan, Y. Zhang, D. K. Rajpal, X. Wu, D. Guo, M. Wang, J.-S. Taylor, and Z. Wang
Specificity of DNA Lesion Bypass by the Yeast DNA Polymerase eta
J. Biol. Chem., March 10, 2000; 275(11): 8233 - 8239.
[Abstract] [Full Text] [PDF]

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 Mol. Cell. Biol.Home page
S. Bärtsch, L. E. Kang, and L. S. Symington
RAD51 Is Required for the Repair of Plasmid Double-Stranded DNA Gaps from Either Plasmid or Chromosomal Templates
Mol. Cell. Biol., February 15, 2000; 20(4): 1194 - 1205.
[Abstract] [Full Text]

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 J. Biol. Chem.Home page
Y. Murakumo, T. Roth, H. Ishii, D. Rasio, S.-i. Numata, C. M. Croce, and R. Fishel
A Human REV7 Homolog That Interacts with the Polymerase zeta Catalytic Subunit hREV3 and the Spindle Assembly Checkpoint Protein hMAD2
J. Biol. Chem., February 11, 2000; 275(6): 4391 - 4397.
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 Cold Spring Harb Symp Quant BiolHome page
V.L. GERLACH, W.J. FEAVER, P.L. FISCHHABER, J.A. RICHARDSON, L. ARAVIND, E.V. KOONIN, K. BEBENEK, T.A. KUNKEL, and E.C. FRIEDBERG
Human DNA Polymerase {kappa}: A Novel DNA Polymerase of Unknown Biological Function Encoded by the DINB1 Gene
Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 41 - 50.
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 Cold Spring Harb Symp Quant BiolHome page
S. PRAKASH, R.E. JOHNSON, M.T. WASHINGTON, L. HARACSKA, C.M. KONDRATICK, and L. PRAKASH
Role of Yeast and Human DNA Polymerase {eta} in Error-free Replication of Damaged DNA
Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 51 - 60.
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 Cold Spring Harb Symp Quant BiolHome page
C.W. LAWRENCE, P.E.M. GIBBS, R.S. MURANTE, X.-D. WANG, Z. LI, T.P. MCMANUS, W.G. MCGREGOR, J.R. NELSON, D.C. HINKLE, and V.M. MAHER
Roles of DNA Polymerase {zeta} and Rev1 Protein in Eukaryotic Mutagenesis and Translesion Replication
Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 61 - 70.
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 Cold Spring Harb Symp Quant BiolHome page
C. MASUTANI, R. KUSUMOTO, A. YAMADA, M. YUASA, M. ARAKI, T. NOGIMORI, M. YOKOI, T. EKI, S. IWAI, and F. HANAOKA
Xeroderma Pigmentosum Variant: From a Human Genetic Disorder to a Novel DNA Polymerase
Cold Spring Harb Symp Quant Biol, January 1, 2000; 65(0): 71 - 80.
[Abstract] [PDF]

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 J. Biol. Chem.Home page
M. T. Washington, R. E. Johnson, S. Prakash, and L. Prakash
Fidelity and Processivity of Saccharomyces cerevisiae DNA Polymerase eta
J. Biol. Chem., December 24, 1999; 274(52): 36835 - 36838.
[Abstract] [Full Text] [PDF]

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 J. Biol. Chem.Home page
N. B. Reuven, G. Arad, A. Maor-Shoshani, and Z. Livneh
The Mutagenesis Protein UmuC Is a DNA Polymerase Activated by UmuD', RecA, and SSB and Is Specialized for Translesion Replication
J. Biol. Chem., November 5, 1999; 274(45): 31763 - 31766.
[Abstract] [Full Text] [PDF]

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 Mol. Cell. Biol.Home page
Y. K. Jang, L. Wang, and G. B. Sancar
RPH1 and GIS1 Are Damage-Responsive Repressors of PHR1
Mol. Cell. Biol., November 1, 1999; 19(11): 7630 - 7638.
[Abstract] [Full Text] [PDF]

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 Proc. Natl. Acad. Sci. USAHome page
R. E. Johnson, M. T. Washington, S. Prakash, and L. Prakash
Bridging the gap: A family of novel DNA polymerases that replicate faulty DNA
PNAS, October 26, 1999; 96(22): 12224 - 12226.
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 Proc. Natl. Acad. Sci. USAHome page
V. L. Gerlach, L. Aravind, G. Gotway, R. A. Schultz, E. V. Koonin, and E. C. Friedberg
Human and mouse homologs of Escherichia coli DinB (DNA polymerase IV), members of the UmuC/DinB superfamily
PNAS, October 12, 1999; 96(21): 11922 - 11927.
[Abstract] [Full Text] [PDF]

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 Genes Dev.Home page
R. Woodgate
A plethora of lesion-replicating DNA polymerases
Genes & Dev., September 1, 1999; 13(17): 2191 - 2195.
[Full Text]

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 Proc. Natl. Acad. Sci. USAHome page
M. Tang, X. Shen, E. G. Frank, M. O'Donnell, R. Woodgate, and M. F. Goodman
UmuD'2C is an error-prone DNA polymerase, Escherichia coli pol V
PNAS, August 3, 1999; 96(16): 8919 - 8924.
[Abstract] [Full Text] [PDF]

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 ScienceHome page
R. E. Johnson, C. M. Kondratick, S. Prakash, and L. Prakash
hRAD30 Mutations in the Variant Form of Xeroderma Pigmentosum
Science, July 9, 1999; 285(5425): 263 - 265.
[Abstract] [Full Text]