An Interspecific Backcross of Lycopersicon esculentum X L. hirsutum: Linkage Analysis and a QTL Study of Sexual Compatibility Factors and Floral Traits

INVESTIGATIONS

An Interspecific Backcross of Lycopersicon esculentum X L. hirsutum: Linkage Analysis and a QTL Study of Sexual Compatibility Factors and Floral Traits

D. Bernacchi and S. D. Tanksley
Department of Plant Breeding and Biometry, Cornell University, Ithaca, New York, 14850

A BC(1) population of the self-compatible tomato Lycopersicon esculentum and its wild self-incompatible relative L. hirsutum f. typicum was used for restriction fragment length polymorphism linkage analysis and quantitative trait loci (QTL) mapping of reproductive behavior and floral traits. The self-incompatibility locus, S, on chromosome 1 harbored the only QTL for self-incompatibility indicating that the transition to self-compatibility in the lineage leading to the cultivated tomato was primarily the result of mutations at the S locus. Moreover, the major QTL controlling unilateral incongruity also mapped to the S locus, supporting the hypothesis that self-incompatibility and unilateral incongruity are not independent mechanisms. The mating behavior of near-isogenic lines carrying the L. hirsutum allele for the S locus on chromosome 1 in an otherwise L. esculentum background support these conclusions. The S locus region of chromosome 1 also harbors most major QTL for several floral traits important to pollination biology (e.g., number and size of flowers), suggesting a gene complex controlling both genetic and morphological mechanisms of reproduction control. Similar associations in other flowering plants suggest that such complex may have been conserved since early periods of plant evolution or else reflect a convergent evolutionary process.

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				A. B. Korol, Y. I. Ronin, A. M. Itskovich, J. Peng, and E. Nevo Enhanced Efficiency of Quantitative Trait Loci Mapping Analysis Based on Multivariate Complexes of Quantitative Traits Genetics, April 1, 2001; 157(4): 1789 - 1803. [Abstract] [Full Text]

				M. E. Nasrallah, K. Yogeeswaran, S. Snyder, and J. B. Nasrallah Arabidopsis Species Hybrids in the Study of Species Differences and Evolution of Amphiploidy in Plants Plant Physiology, December 1, 2000; 124(4): 1605 - 1614. [Abstract] [Full Text]

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				R. T. Chetelat, V. Meglic, and P. Cisneros A Genetic Map of Tomato Based on BC1 Lycopersicon esculentum Solanum lycopersicoides Reveals Overall Synteny but Suppressed Recombination Between These Homeologous Genomes Genetics, February 1, 2000; 154(2): 857 - 867. [Abstract] [Full Text]

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				G. Suzuki, N. Kai, T. Hirose, K. Fukui, T. Nishio, S. Takayama, A. Isogai, M. Watanabe, and K. Hinata Genomic Organization of the S Locus: Identification and Characterization of Genes in SLG/SRK Region of S9 Haplotype of Brassica campestris (syn. rapa) Genetics, September 1, 1999; 153(1): 391 - 400. [Abstract] [Full Text]

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