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Genetics, Vol 147, 589-596, Copyright © 1997
INVESTIGATIONS |
Homology-Dependent Silencing of the SC3 Gene in Schizophyllum commune
T. A. Schuurs, EAM. Schaeffer and JGH. Wessels
Department of Plant Biology, Groningen Biomolecular Sciences and Technology Institute, University of Groningen, 9751 NN, Haren, The Netherlands
After introduction of extra copies of the SC3 hydrophobin gene into a wild-type strain of Schizophyllum commune, gene silencing was observed acting on both endogenous and introduced SC3 genes in primary vegetative transformants. Nuclear run-on experiments indicated that silencing acted at the transcriptional level. Southern analysis revealed that cytosine methylation of genomic DNA occurred. Moreover, SC3 silencing was suppressed by exposure to 5-azacytidine during growth. After growth of SC3-suppressed colonies from homogenized mycelium or from colonies stored at 4{deg}, SC3 transcription was restored. However, after prolonged growth SC3 silencing was again observed. Introduction of a promoterless SC3 fragment into wild type gave less SC3 silencing.
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