Genetics, Vol 147, 87-100, Copyright © 1997

INVESTIGATIONS

In Vivo Analysis of Saccharomyces cerevisiae COX2 mRNA 5'-Untranslated Leader Functions in Mitochondrial Translation Initiation and Translational Activation

H. M. Dunstan, N. S. Green-Willms and T. D. Fox
Section of Genetics and Development, Cornell University, Ithaca, New York 14853-2703

We have used mutational and revertant analysis to study the elements of the 54-nucleotide COX2 5'-untranslated leader involved in translation initiation in yeast mitochondria and in activation by the COX2 translational activator, Pet111p. We generated a collection of mutants with substitutions spanning the entire COX2 5'-UTL by in vitro mutagenesis followed by mitochondrial transformation and gene replacement. The phenotypes of these mutants delimit a 31-nucleotide segment, from -16 to -46, that contains several short sequence elements necessary for COX2 5'-UTL function in translation. The sequences from -16 to -47 were shown to be partially sufficient to promote translation in a foreign context. Analysis of revertants of both the series of linker-scanning alleles and two short deletion/insertion alleles has refined the positions of several possible functional elements of the COX2 5'-untranslated leader, including a putative RNA stem-loop structure that functionally interacts with Pet111p and an octanucleotide sequence present in all S. cerevisiae mitochondrial mRNA 5'-UTLs that is a potential rRNA binding site.

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