- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Clark, A. G.
- Articles by Wang, L.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Clark, A. G.
- Articles by Wang, L.
Genetics, Vol 147, 157-163, Copyright © 1997
INVESTIGATIONS |
Epistasis in Measured Genotypes: Drosophila P-Element Insertions
A. G. Clark and L. Wang
Institute of Molecular Evolutionary Genetics, Department of Biology, Pennsylvania State University, University Park, Pennsylvania 16802
Transposon tagging provides an opportunity to construct large numbers of strains of organisms that differ by single insertional mutations. By scoring the phenotypes of these ``measured genotypes,'' powerful tests of effects of mutations on phenotypic expression have been performed. Here we extend this approach by constructing with simple crosses all possible two-locus genotypes for each of eight pairs of P-element insertions. Analysis of metabolic phenotypes (fat and glycogen contents, enzyme activities, total protein, and body weight) of the resulting nine genotypes provides direct estimates of additive, dominance, and epistatic effects of the mutations. Nested two-way analysis of variance identified significant epistatic effects in 27% of the tests (35/128 of the trait X P-element combinations). Posterior contrasts were performed to partition the epistatic variance into the four orthogonal components of COCKERHAM, and the data exhibit a tendency toward additive X dominance and dominance X dominance epistasis. Mutations in this study have epistatic effects on metabolic traits that are on the same order of magnitude as main (additive and dominance) effects. Measured genotypes have been used in other contexts to quantify epistatic effects on phenotypic expression, and these results are also briefly reviewed.
This article has been cited by other articles:
 |
|
 |
|
  D. L. Crawford and M. F. Oleksiak The biological importance of measuring individual variation J. Exp. Biol., May 1, 2007; 210(9): 1613 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. K. Kelly Epistasis in Monkeyflowers Genetics, December 1, 2005; 171(4): 1917 - 1931. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. van Swinderen and R. J. Greenspan Flexibility in a Gene Network Affecting a Simple Behavior in Drosophila melanogaster Genetics, April 1, 2005; 169(4): 2151 - 2163. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Lauter, C. Gustus, A. Westerbergh, and J. Doebley The Inheritance and Evolution of Leaf Pigmentation and Pubescence in Teosinte Genetics, August 1, 2004; 167(4): 1949 - 1959. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. L. Montooth, J. H. Marden, and A. G. Clark Mapping Determinants of Variation in Energy Metabolism, Respiration and Flight in Drosophila Genetics, October 1, 2003; 165(2): 623 - 635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. F. Hansen and G. P. Wagner Epistasis and the Mutation Load: A Measurement-Theoretical Approach Genetics, May 1, 2001; 158(1): 477 - 485. [Abstract] [Full Text]
|
|
|
|
|
|
B. Bost, C. Dillmann, and D. de Vienne Fluxes and Metabolic Pools as Model Traits for Quantitative Genetics. I. The L-Shaped Distribution of Gene Effects Genetics, December 1, 1999; 153(4): 2001 - 2012. [Abstract] [Full Text]
|
|
|
|
|
|
U. Bhadra, M. P. Bhadra, and J. A. Birchler Interactions Among Dosage-Dependent Trans-Acting Modifiers of Gene Expression and Position-Effect Variegation in Drosophila Genetics, September 1, 1998; 150(1): 251 - 263. [Abstract] [Full Text]
|
|
|
|
|
|
P. C. Phillips The Language of Gene Interaction Genetics, July 1, 1998; 149(3): 1167 - 1171. [Full Text] [PDF]
|
|