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Genetics, Vol 146, 859-869, Copyright © 1997
INVESTIGATIONS |
Mating Type in Chlamydomonas Is Specified by mid, the Minus-Dominance Gene
P. J. Ferris and U. W. Goodenough
Department of Biology, Washington University, St. Louis, Missouri 63130
Diploid cells of Chlamydomonas reinhardtii that are heterozygous at the mating-type locus (mt(+)/mt(-)) differentiate as minus gametes, a phenomenon known as minus dominance. We report the cloning and characterization of a gene that is necessary and sufficient to exert this minus dominance over the plus differentiation program. The gene, called mid, is located in the rearranged (R) domain of the mt(-) locus, and has duplicated and transposed to an autosome in a laboratory strain. The imp11 mt(-) mutant, which differentiates as a fusion-incompetent plus gamete, carries a point mutation in mid. Like the fus1 gene in the mt(+) locus, mid displays low codon bias compared with other nuclear genes. The mid sequence carries a putative leucine zipper motif, suggesting that it functions as a transcription factor to switch on the minus program and switch off the plus program of gametic differentiation. This is the first sex-determination gene to be characterized in a green organism.
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