- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by McKee, AHZ.
- Articles by Kleckner, N.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by McKee, AHZ.
- Articles by Kleckner, N.
Genetics, Vol 146, 817-834, Copyright © 1997
INVESTIGATIONS |
Mutations in Saccharomyces cerevisiae That Block Meiotic Prophase Chromosome Metabolism and Confer Cell Cycle Arrest at Pachytene Identify Two New Meiosis-Specific Genes SAE1 and SAE3
AHZ. McKee and N. Kleckner
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138
Two new meiosis-specific genes, SAE1 and SAE3, have been identified in a screen for mutations that confer an intermediate block in meiotic prophase. Such mutations confer a block to spore formation that is circumvented by addition of a mutation that eliminates meiotic recombination initiation and other aspects of chromosome metabolism, i.e., spo11. We show that sae1-1 and sae3-1 mutations each confer a distinct defect in meiotic recombination. sae1-1 produces recombinants but very slowly and ultimately to less than half the wild-type level; sae3-1 makes persistent hyper-resected meiotic double-strand breaks and has a severe defect in formation of recombinants. Both mutants arrest at the pachytene stage of meiotic prophase, sae1-1 temporarily and sae3-1 permanently. The phenotypes conferred by sae3-1 are similar to those conferred by mutation of the yeast RecA homologue DMC1, suggesting that SAE3 and DMC1 act at the same step(s) of chromosome metabolism. These results provide further evidence that intermediate blocks to prophase chromosome metabolism cause cell-cycle arrest. SAE1 encodes a 208-residue protein homologous to vertebrate mRNA cap-binding protein 20. SAE3 corresponds to a meiosis-specific RNA encoding an unusually short open reading frame of 50 codons.
This article has been cited by other articles:
 |
|
 |
|
  J. C. Mell, B. L. Wienholz, A. Salem, and S. M. Burgess Sites of Recombination Are Local Determinants of Meiotic Homolog Pairing in Saccharomyces cerevisiae Genetics, June 1, 2008; 179(2): 773 - 784. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Ellermeier, H. Schmidt, and G. R. Smith Swi5 Acts in Meiotic DNA Joint Molecule Formation in Schizosaccharomyces pombe Genetics, December 1, 2004; 168(4): 1891 - 1898. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Tsubouchi and G. S. Roeder The Budding Yeast Mei5 and Sae3 Proteins Act Together With Dmc1 During Meiotic Recombination Genetics, November 1, 2004; 168(3): 1219 - 1230. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. A. Young, R. W. Hyppa, and G. R. Smith Conserved and Nonconserved Proteins for Meiotic DNA Breakage and Repair in Yeasts Genetics, June 1, 2004; 167(2): 593 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Akamatsu, D. Dziadkowiec, M. Ikeguchi, H. Shinagawa, and H. Iwasaki Two different Swi5-containing protein complexes are involved in mating-type switching and recombination repair in fission yeast PNAS, December 23, 2003; 100(26): 15770 - 15775. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.-J. E. Hong and G. S. Roeder A role for Ddc1 in signaling meiotic double-strand breaks at the pachytene checkpoint Genes & Dev., February 1, 2002; 16(3): 363 - 376. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. de los Santos, J. Loidl, B. Larkin, and N. M. Hollingsworth A Role for MMS4 in the Processing of Recombination Intermediates During Meiosis in Saccharomyces cerevisiae Genetics, December 1, 2001; 159(4): 1511 - 1525. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Tsubouchi and H. Ogawa Exo1 Roles for Repair of DNA Double-Strand Breaks and Meiotic Crossing Over in Saccharomyces cerevisiae Mol. Biol. Cell, July 1, 2000; 11(7): 2221 - 2233. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Paques and J. E. Haber Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae Microbiol. Mol. Biol. Rev., June 1, 1999; 63(2): 349 - 404. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. R. Hepworth, H. Friesen, and J. Segall NDT80 and the Meiotic Recombination Checkpoint Regulate Expression of Middle Sporulation-Specific Genes in Saccharomyces cerevisiae Mol. Cell. Biol., October 1, 1998; 18(10): 5750 - 5761. [Abstract] [Full Text]
|
|
|
|
|
|
J. Engebrecht, S. Masse, L. Davis, K. Rose, and T. Kessel Yeast Meiotic Mutants Proficient for the Induction of Ectopic Recombination Genetics, February 1, 1998; 148(2): 581 - 598. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. S. Roeder Meiotic chromosomes: it takes two to tango Genes & Dev., October 15, 1997; 11(20): 2600 - 2621. [Full Text] [PDF]
|
|