- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Morgan, L. W.
- Articles by Feldman, J. F.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Morgan, L. W.
- Articles by Feldman, J. F.
Genetics, Vol 146, 525-530, Copyright © 1997
INVESTIGATIONS |
Isolation and Characterization of a Temperature-Sensitive Circadian Clock Mutant of Neurospora crassa
L. W. Morgan and J. F. Feldman
Department of Biology, University of California, Santa Cruz, Santa Cruz, California 95064
A new circadian clock mutant has been isolated in Neurospora crassa. This new mutation, called period-6 (prd-6), has two features novel to known clock mutations. First, the mutation is temperature sensitive. At restrictive temperatures (above 21{deg}) the mutation shortens circadian period length from a wild-type value of 21.5 hr to 18 hr. At permissive temperatures (below 21{deg}) the mutant has a 20.5-hr period length close to that of the wild-type strain. Second, the prd-6 mutation is epistatic to the previously isolated clock mutation period-2 (prd-2). This epistasis is unusual in that the prd-2 prd-6 double mutant strain has an 18-hr period length at both the restrictive and permissive temperatures. That is, the temperature-sensitive aspect of the phenotype of the prd-6 strain is lost in the prd-2 prd-6 double mutant strain. This suggests that the gene products of the prd-2 and prd-6 loci may interact physically and that the presence of a normal prd-2(+) protein is required for low temperature to ``rescue'' the prd-6 mutant phenotype. These results, combined with our recent finding that prd-2 and some alleles of the frq gene show genetic synergy, suggest that it may be possible to establish a more comprehensive model of the Neurospora circadian clock.
This article has been cited by other articles:
 |
|
 |
|
  L. W. Morgan and J. F. Feldman Epistatic and Synergistic Interactions Between Circadian Clock Mutations in Neurospora crassa Genetics, October 1, 2001; 159(2): 537 - 543. [Abstract] [Full Text] [PDF]
|
|