- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Meirelles, F. V.
- Articles by Smith, L. C.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Meirelles, F. V.
- Articles by Smith, L. C.
Genetics, Vol 145, 445-451, Copyright © 1997
INVESTIGATIONS |
Mitochondrial Genotype Segregation in a Mouse Heteroplasmic Lineage Produced by Embryonic Karyoplast Transplantation
F. V. Meirelles and L. C. Smith
Centre de recherche en reproduction animale, Faculte de medecine veterinaire, Universite de Montreal, Saint-Hyacinthe, Quebec J2S 7C6, Canada
Mitochondrial genotypes have been shown to segregate both rapidly and slowly when transmitted to consecutive generations in mammals. Our objective was to develop an animal model to analyze the patterns of mammalian mitochondrial DNA (mtDNA) segregation and transmission in an intraspecific heteroplasmic maternal lineage to investigate the mechanisms controlling these phenomena. Heteroplasmic progeny were obtained from reconstructed blastocysts derived by transplantation of pronuclear-stage karyoplasts to enucleated zygotes with different mtDNA. Although the reconstructed zygotes contained on average 19% mtDNA of karyoplast origin, most progeny contained fewer mtDNA of karyoplast origin and produced exclusively homoplasmic first generation progeny. However, one founder heteroplasmic adult female had elevated tissue heteroplasmy levels, varying from 6% (lung) to 69% (heart), indicating that stringent replicative segregation had occurred during mitotic divisions. First generation progeny from the above female were all heteroplasmic, indicating that, despite a meiotic segregation, they were derived from heteroplasmic founder oocytes. Some second and third generation progeny contained exclusively New Zealand Black/BINJ mtDNA, suggesting, but not confirming, an origin from an homoplasmic oocyte. Moreover, several third to fifth generation individuals maintained mtDNA from both mouse strains, indicating a slow or persistent segregation pattern characterized by diminished tissue and litter variability beyond second generation progeny. Therefore, although some initial lineages appear to segregate rapidly to homoplasmy, within two generations other lineages transmit stable amounts of both mtDNA molecules, supporting a mechanism where mitochondria of different origin may fuse, leading to persistent intraorganellar heteroplasmy.
This article has been cited by other articles:
 |
|
 |
|
  B.M Acton, I Lai, X Shang, A Jurisicova, and R.F Casper Neutral Mitochondrial Heteroplasmy Alters Physiological Function in Mice Biol Reprod, September 1, 2007; 77(3): 569 - 576. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E.C. Spikings, J. Alderson, and J.C.St. John Transmission of mitochondrial DNA following assisted reproduction and nuclear transfer Hum. Reprod. Update, July 1, 2006; 12(4): 401 - 415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. E. Lloyd, J.-H. Lee, R. Alberio, E. J. Bowles, J. Ramalho-Santos, K. H. S. Campbell, and J. C. St. John Aberrant Nucleo-cytoplasmic Cross-Talk Results in Donor Cell mtDNA Persistence in Cloned Embryos Genetics, April 1, 2006; 172(4): 2515 - 2527. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.J.A.M. Jacobs, G. de Wert, J.P.M. Geraedts, I.F.M. de Coo, and H.J.M. Smeets The transmission of OXPHOS disease and methods to prevent this Hum. Reprod. Update, March 1, 2006; 12(2): 119 - 136. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hamatani, G. Falco, M. G. Carter, H. Akutsu, C. A. Stagg, A. A. Sharov, D. B. Dudekula, V. VanBuren, and M. S.H. Ko Age-associated alteration of gene expression patterns in mouse oocytes Hum. Mol. Genet., October 1, 2004; 13(19): 2263 - 2278. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C St John, R. E I Lloyd, E. J Bowles, E. C Thomas, and S. El Shourbagy The consequences of nuclear transfer for mammalian foetal development and offspring survival. A mitochondrial DNA perspective Reproduction, June 1, 2004; 127(6): 631 - 641. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. C. St. John and G. Schatten Paternal Mitochondrial DNA Transmission During Nonhuman Primate Nuclear Transfer Genetics, June 1, 2004; 167(2): 897 - 905. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. McKenzie, I. A. Trounce, C. A. Cassar, and C. A. Pinkert Production of homoplasmic xenomitochondrial mice PNAS, February 10, 2004; 101(6): 1685 - 1690. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. L. Dean, B. J. Battersby, A. Ao, R. G. Gosden, S. L. Tan, and E. A. Shoubridge Prospect of preimplantation genetic diagnosis for heritable mitochondrial DNA diseases Mol. Hum. Reprod., October 1, 2003; 9(10): 631 - 638. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P F Chinnery and E A Schon Mitochondria J. Neurol. Neurosurg. Psychiatry, September 1, 2003; 74(9): 1188 - 1199. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hiendleder, V. Zakhartchenko, H. Wenigerkind, H.-D. Reichenbach, K. Bruggerhoff, K. Prelle, G. Brem, M. Stojkovic, and E. Wolf Heteroplasmy in Bovine Fetuses Produced by Intra- and Inter-Subspecific Somatic Cell Nuclear Transfer: Neutral Segregation of Nuclear Donor Mitochondrial DNA in Various Tissues and Evidence for Recipient Cow Mitochondria in Fetal Blood Biol Reprod, January 1, 2003; 68(1): 159 - 166. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Steinborn, P. Schinogl, D. N. Wells, A. Bergthaler, M. Muller, and G. Brem Coexistence of Bos taurus and B. indicus Mitochondrial DNAs in Nuclear Transfer-Derived Somatic Cattle Clones Genetics, October 1, 2002; 162(2): 823 - 829. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. C. St. John Ooplasm donation in humans: The need to investigate the transmission of mitochondrial DNA following cytoplasmic transfer Hum. Reprod., August 1, 2002; 17(8): 1954 - 1958. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. V. Meirelles, V. Bordignon, Y. Watanabe, M. Watanabe, A. Dayan, R. B. Lôbo, J. M. Garcia, and L. C. Smith Complete Replacement of the Mitochondrial Genotype in a Bos indicus Calf Reconstructed by Nuclear Transfer to a Bos taurus Oocyte Genetics, May 1, 2001; 158(1): 351 - 356. [Abstract] [Full Text]
|
|
|
|
|
|
D. Mehmet, F. Ahmed, J.M. Cummins, R. Martin, and J. Whelan Quantification of the common deletion in human testicular mitochondrial DNA by competitive PCR assay using a chimaeric competitor Mol. Hum. Reprod., March 1, 2001; 7(3): 301 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Shitara, H. Kaneda, A. Sato, K. Inoue, A. Ogura, H. Yonekawa, and J.-I. Hayashi Selective and Continuous Elimination of Mitochondria Microinjected Into Mouse Eggs From Spermatids, but Not From Liver Cells, Occurs Throughout Embryogenesis Genetics, November 1, 2000; 156(3): 1277 - 1284. [Abstract] [Full Text]
|
|
|
|
|
|
K. Takeda, S. Takahashi, A. Onishi, H. Hanada, and H. Imai Replicative Advantage and Tissue-Specific Segregation of RR Mitochondrial DNA Between C57BL/6 and RR Heteroplasmic Mice Genetics, June 1, 2000; 155(2): 777 - 783. [Abstract] [Full Text]
|
|
|
|
 |
|
 D. C. Wallace Mitochondrial Diseases in Man and Mouse Science, March 5, 1999; 283(5407): 1482 - 1488. [Abstract] [Full Text]
|
|
|
|
|
|
F. V. Meirelles and L. C. Smith Mitochondrial Genotype Segregation During Preimplantation Development in Mouse Heteroplasmic Embryos Genetics, February 1, 1998; 148(2): 877 - 884. [Abstract] [Full Text] [PDF]
|
|