Genetics, Vol 144, 1757-1767, Copyright © 1996

INVESTIGATIONS

Genetic and Physical Mapping of the Mouse Ulnaless Locus

C. L. Peichel, C. M. Abbott and T. F. Vogt
Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544

The mouse Ulnaless locus is a semidominant mutation which displays defects in patterning along the proximal-distal and anterior-posterior axes of all four limbs. The first Ulnaless homozygotes have been generated, and they display a similar, though slightly more severe, limb phenotype than the heterozygotes. To create a refined genetic map of the Ulnaless region using molecular markers, four backcrosses segregating Ulnaless were established. A 0.4-cM interval containing the Ulnaless locus has been defined on mouse chromosome 2, which has identified Ulnaless as a possible allele of a Hoxd cluster gene(s). With this genetic map as a framework, a physical map of the Ulnaless region has been completed. Yeast artificial chromosomes covering this region have been isolated and ordered into a 2 Mb contig. Therefore, the region that must contain the Ulnaless locus has been defined and cloned, which will be invaluable for the identification of the molecular nature of the Ulnaless mutation.

This article has been cited by other articles:

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				C. Peichel, B Prabhakaran, and T. Vogt The mouse Ulnaless mutation deregulates posterior HoxD gene expression and alters appendicular patterning Development, January 9, 1997; 124(18): 3481 - 3492. [Abstract] [PDF]

				Y Herault, N Fraudeau, J Zakany, and D Duboule Ulnaless (Ul), a regulatory mutation inducing both loss-of-function and gain-of-function of posterior Hoxd genes Development, January 9, 1997; 124(18): 3493 - 3500. [Abstract] [PDF]