The Autosomal FLP-DFS Technique for Generating Germline Mosaics in Drosophila melanogaster

INVESTIGATIONS

The Autosomal FLP-DFS Technique for Generating Germline Mosaics in Drosophila melanogaster

T. B. Chou and N. Perrimon
Present address: Department of Zoology, National Taiwan University, Taipei, Taiwan.

The production of female germline chimeras is invaluable for analyzing the tissue specificity of recessive female sterile mutations as well as detecting the maternal effect of recessive zygotic lethal mutations. Previously, we developed the ``FLP-DFS'' technique to efficiently generate germline clones. This technique uses the X-linked germline-dependent dominant female sterile mutation ovo(D1) as a selection for the detection of germline recombination events, and the FLP-FRT recombination system to promote site-specific chromosomal exchange. This method allows the efficient production of germline mosaics only on the X chromosome. In this paper we have built chromosomes that allow the use of this technique to the autosomes. We describe the various steps involved in the development of this technique as well as the properties of the chromosomes utilized.

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				R. M. Goodman, S. Thombre, Z. Firtina, D. Gray, D. Betts, J. Roebuck, E. P. Spana, and E. M. Selva Sprinter: a novel transmembrane protein required for Wg secretion and signaling Development, December 15, 2006; 133(24): 4901 - 4911. [Abstract] [Full Text] [PDF]

				S. Simoes, B. Denholm, D. Azevedo, S. Sotillos, P. Martin, H. Skaer, J. C.-G. Hombria, and A. Jacinto Compartmentalisation of Rho regulators directs cell invagination during tissue morphogenesis Development, November 1, 2006; 133(21): 4257 - 4267. [Abstract] [Full Text] [PDF]

				N. Vogt, I. Koch, H. Schwarz, F. Schnorrer, and C. Nusslein-Volhard The {gamma}TuRC components Grip75 and Grip128 have an essential microtubule-anchoring function in the Drosophila germline Development, October 15, 2006; 133(20): 3963 - 3972. [Abstract] [Full Text] [PDF]

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				S. Polaski, L. Whitney, B. W. Barker, and B. Stronach Genetic Analysis of Slipper/Mixed Lineage Kinase Reveals Requirements in Multiple Jun-N-Terminal Kinase-Dependent Morphogenetic Events During Drosophila Development Genetics, October 1, 2006; 174(2): 719 - 733. [Abstract] [Full Text] [PDF]

				C. Laplante and L. A. Nilson Differential expression of the adhesion molecule Echinoid drives epithelial morphogenesis in Drosophila Development, August 15, 2006; 133(16): 3255 - 3264. [Abstract] [Full Text] [PDF]

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				A. M. O'Reilly, A. C. Ballew, B. Miyazawa, H. Stocker, E. Hafen, and M. A. Simon Csk differentially regulates Src64 during distinct morphological events in Drosophila germ cells Development, July 15, 2006; 133(14): 2627 - 2638. [Abstract] [Full Text] [PDF]

				B. P. Levi, A. S. Ghabrial, and M. A. Krasnow Drosophila talin and integrin genes are required for maintenance of tracheal terminal branches and luminal organization Development, June 15, 2006; 133(12): 2383 - 2393. [Abstract] [Full Text] [PDF]

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				D. Kent, E. W. Bush, and J. E. Hooper Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus. Development, May 1, 2006; 133(10): 2001 - 2010. [Abstract] [Full Text] [PDF]

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