Contrasting Histories of Three Gene Regions Associated With In(3L)Payne of Drosophila melanogaster

INVESTIGATIONS

Contrasting Histories of Three Gene Regions Associated With In(3L)Payne of Drosophila melanogaster

E. Hasson and W. F. Eanes
Present address: GIBE, Departamento de Ciencias Biologicas, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Ciudad Universitaria Pab. II. (1428), Buenos Aires, Argentina.

In the present report, we studied nucleotide variation in three gene regions of Drosophila melanogaster, spanning >5 kb and showing different degrees of association with the cosmopolitan inversion In(3-L)Payne. The analysis of sequence variation in the regions surrounding the breakpoints and the heat shock 83 (Hsp83) gene locus, located close to the distal breakpoint, revealed the absence of shared polymorphisms and the presence of a number of fixed differences between arrangements, indicating absence of genetic exchange. In contrast, for the esterase-6 gene region, located in the center of the inversion, we observed the presence of shared polymorphisms between arrangements suggesting genetic exchange. In the regions close to the breakpoints, the common St arrangement is 10 times more polymorphic than inverted chromosomes. We propose that the lack of recombination between arrangements in these regions coupled with genetic hitchhiking is the best explanation for the low heterozygosity observed in inverted lines. Using the data for the breakpoints, we estimate that this inversion polymorphism is around 0.36 million yr old. Although it is widely accepted that inversions are examples of balanced polymorphisms, none of the current neutrality tests including our Monte Carlo simulations showed significant departure from neutral expectations.

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				A. Navarro-Sabate, M. Aguade, and C. Segarra Excess of Nonsynonymous Polymorphism at Acph-1 in Different Gene Arrangements of Drosophila subobscura Mol. Biol. Evol., November 1, 2003; 20(11): 1833 - 1843. [Abstract] [Full Text] [PDF]

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				F. Casals, M. Caceres, and A. Ruiz The Foldback-like Transposon Galileo Is Involved in the Generation of Two Different Natural Chromosomal Inversions of Drosophila buzzatii Mol. Biol. Evol., May 1, 2003; 20(5): 674 - 685. [Abstract] [Full Text] [PDF]

				G. A. Gomez and E. Hasson Transpecific Polymorphisms in an Inversion Linked Esterase Locus in Drosophila buzzatii Mol. Biol. Evol., March 1, 2003; 20(3): 410 - 423. [Abstract] [Full Text] [PDF]

				A. G. Saez, A. Tatarenkov, E. Barrio, N. H. Becerra, and F. J. Ayala Patterns of DNA sequence polymorphism at Sod vicinities in Drosophila melanogaster: Unraveling the footprint of a recent selective sweep PNAS, February 18, 2003; 100(4): 1793 - 1798. [Abstract] [Full Text] [PDF]

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				M. Kauer, B. Zangerl, D. Dieringer, and C. Schlotterer Chromosomal Patterns of Microsatellite Variability Contrast Sharply in African and Non-African Populations of Drosophila melanogaster Genetics, January 1, 2002; 160(1): 247 - 256. [Abstract] [Full Text] [PDF]

				B. C. Verrelli and W. F. Eanes Extensive Amino Acid Polymorphism at the Pgm Locus Is Consistent With Adaptive Protein Evolution in Drosophila melanogaster Genetics, December 1, 2000; 156(4): 1737 - 1752. [Abstract] [Full Text]

				M. Kovacevic and S. W. Schaeffer Molecular Population Genetics of X-Linked Genes in Drosophila pseudoobscura Genetics, September 1, 2000; 156(1): 155 - 172. [Abstract] [Full Text]

				P. Andolfatto and M. Przeworski A Genome-Wide Departure From the Standard Neutral Model in Natural Populations of Drosophila Genetics, September 1, 2000; 156(1): 257 - 268. [Abstract] [Full Text]

				A. Navarro, A. Barbadilla, and A. Ruiz Effect of Inversion Polymorphism on the Neutral Nucleotide Variability of Linked Chromosomal Regions in Drosophila Genetics, June 1, 2000; 155(2): 685 - 698. [Abstract] [Full Text]

				P. Andolfatto, J. D. Wall, and M. Kreitman Unusual Haplotype Structure at the Proximal Breakpoint of In(2L)t in a Natural Population of Drosophila melanogaster Genetics, November 1, 1999; 153(3): 1297 - 1311. [Abstract] [Full Text]

				E. S. Balakirev, E. I. Balakirev, F. Rodríguez-Trelles, and F. J. Ayala Molecular Evolution of Two Linked Genes, Est-6 and Sod, in Drosophila melanogaster Genetics, November 1, 1999; 153(3): 1357 - 1369. [Abstract] [Full Text]

				F. Depaulis, L. Brazier, and M. Veuille Selective Sweep at the Drosophila melanogaster Suppressor of Hairless Locus and Its Association With the In(2L)t Inversion Polymorphism Genetics, July 1, 1999; 152(3): 1017 - 1024. [Abstract] [Full Text]

				J. Rozas, C. Segarra, G. Ribó, and M. Aguadé Molecular Population Genetics of the rp49 Gene Region in Different Chromosomal Inversions of Drosophila subobscura Genetics, January 1, 1999; 151(1): 189 - 202. [Abstract] [Full Text]

				M. Caceres, M. Puig, and A. Ruiz Molecular Characterization of Two Natural Hotspots in the Drosophila buzzatii Genome Induced by Transposon Insertions Genome Res., August 1, 2001; 11(8): 1353 - 1364. [Abstract] [Full Text] [PDF]