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Genetics, Vol 144, 1529-1544, Copyright © 1996
INVESTIGATIONS |
Repression of Hybrid Dysgenesis in Drosophila melanogaster by Heat-Shock-Inducible Sense and Antisense P-Element Constructs
M. J. Simmons, J. D. Raymond, C. D. Grimes, C. Belinco, B. C. Haake, M. Jordan, C. Lund, T. A. Ojala and D. Papermaster
Department of Genetics and Cell Biology, University of Minnesota, St. Paul, Minnesota 55108-1095
Sets of sense and antisense P-element constructs controlled by a heat-shock-inducible promoter were tested for their ability to repress manifestations of P-element activity in vivo. As a group, the antisense constructs repressed pupal lethality, a somatic manifestation of P activity, and this repression was significantly enhanced by heat shock. Three of the 11 antisense constructs also repressed gonadal dysgenesis, a manifestation of P activity in the female germ line; however, none had any effect on P-element-mediated mutability in the male germ line. Among the 13 different heat-shock-inducible sense constructs that were tested, those containing the KP and DP elements were strong repressors of pupal lethality, gonadal dysgenesis and P-element-mediated mutability; however, individual lines carrying these constructs varied in their ability to repress each of these traits, presumably because of genomic position effects. With the exception of the sense construct that contained a complete P element, none of the sense or antisense constructs repressed a lacZ reporter gene driven by the P-element promoter. Overall, the experimental results suggest that in nature, P-element activity could be regulated by P-encoded polypeptides and by antisense P RNAs.
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