- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Allen, S. L.
- Articles by Orias, E.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Allen, S. L.
- Articles by Orias, E.
Genetics, Vol 144, 1489-1496, Copyright © 1996
INVESTIGATIONS |
Mapping Three Classical Isozyme Loci in Tetrahymena: Meiotic Linkage of EstA to the ChxA Linkage Group
S. L. Allen, D. Zeilinger and E. Orias
Department of Biology, University of Michigan, Ann Arbor, Michigan 48109-1048
We demonstrate a reliable method for mapping conventional loci and obtaining meiotic linkage data for the ciliated protozoan Tetrahymena thermophila. By coupling nullisomic deletion mapping with meiotic linkage mapping, loci known to be located on a particular chromosome or chromosome arm can be tested for recombination. This approach has been used to map three isozyme loci, EstA (Esterase A), EstB (Esterase B), and AcpA (Acid Phosphatase A), with respect to the ChxA locus (cycloheximide resistance) and 11 RAPDs (randomly amplified polymorphic DNAs). To assign isozyme loci to chromosomes, clones of inbred strains C3 or C2 were crossed to inbred strain B nullisomics. EstA, EstB and AcpA were mapped to chromosomes 1R, 3L and 3R, respectively. To test EstA and AcpA for linkage to known RAPD loci on their respective chromosomes, a panel of Round II (genomic exclusion) segregants from a B/C3 heterozygote was used. Using the MAPMAKER program, EstA was assigned to the ChxA linkage group on chromosome 1R, and a detailed map was constructed that includes 10 RAPDs. AcpA (on 3R), while unlinked to all the RAPDs assigned to chromosome 3 by nullisomic mapping, does show linkage to a RAPD not yet assignable to chromosomes by nullisomic mapping.
This article has been cited by other articles:
 |
|
 |
|
  E. Hamilton, P. Bruns, C. Lin, V. Merriam, E. Orias, L. Vong, and D. Cassidy-Hanley Genome-Wide Characterization of Tetrahymena thermophila Chromosome Breakage Sites. I. Cloning and Identification of Functional Sites Genetics, August 1, 2005; 170(4): 1611 - 1621. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Orias Mapping the Germ-Line and Somatic Genomes of a Ciliated Protozoan, Tetrahymena thermophila Genome Res., February 1, 1998; 8(2): 91 - 99. [Abstract] [Full Text]
|
|