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Genetics, Vol 144, 1437-1444, Copyright © 1996
INVESTIGATIONS |
Deletion of the Schizophyllum commune A{alpha} Locus: The Roles of A{alpha} Y and Z Mating-Type Genes
C. I. Robertson, K. A. Bartholomew, C. P. Novotny and R. C. Ullrich
Cell and Molecular Biology Program, Department of Botany and Agricultural Biochemistry, University of Vermont, Burlington, Vermont 05405
The A{alpha} locus is one of four master regulatory loci that determine mating type and regulate sexual development in Schizophyllum commune. We have made a plasmid containing a URA1 gene disruption of the A{alpha} Y1 gene. Y1 is the sole A{alpha} gene in A{alpha}1 strains. We used the plasmid construction to produce an A{alpha} null (i.e., A{alpha}{Delta}) strain by replacing the genomic Y1 gene with URA1 in an A{alpha}1 strain. To characterize the role of the A{alpha} genes in the regulation of sexual development, we transformed various A{alpha} Y and Z alleles into A{alpha}{Delta} strains and examined the acquired mating types and mating abilities of the transformants. These experiments demonstrate that the A{alpha} Y gene is not essential for fungal viability and growth, that a solitary Z A{alpha} mating-type gene does not itself activate development, that A{beta} proteins are sufficient to activate the A developmental pathway in the absence of A{alpha} proteins and confirm that Y and Z genes are the sole determinants of A{alpha} mating type. The data from these experiments support and refine our model of the regulation of A-pathway events by Y and Z proteins.
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  C. I. Robertson, A. McMahon Kende, K. Toenjes, C. P. Novotny, and R. C. Ullrich Evidence for Interaction of Schizophyllum commune Y Mating-Type Proteins in Vivo Genetics, April 1, 2002; 160(4): 1461 - 1467. [Abstract] [Full Text] [PDF]
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