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Genetics, Vol 144, 905-921, Copyright © 1996
INVESTIGATIONS |
Identification and Characterization of FAR3, a Gene Required for Pheromone-Mediated G(1) Arrest in Saccharomyces cerevisiae
J. Horecka and G. F. Sprague-Jr.
Present address: National Institute of Bioscience and Human Technology, AIST, Higashi 1-1, Tsukuba, Ibaraki 305, Japan.
In haploid Saccharomyces cerevisiae cells, mating pheromones activate a signal transduction pathway that leads to cell cycle arrest in the G(1) phase and to transcription induction of genes that promote conjugation. To identify genes that link the signal transduction pathway and the cell cycle machinery, we developed a selection strategy to isolate yeast mutants specifically defective for G(1) arrest. Several of these mutants identified previously known genes, including CLN3, FUS3, and FAR1. In addition, a new gene, FAR3, was identified and characterized. FAR3 encodes a novel protein of 204 amino acid residues that is dispensable for viability. Northern blot experiments indicated that FAR3 expression is constitutive with respect to cell type, pheromone treatment, and cell cycle position. As a first step toward elucidating the mechanism by which Far3 promotes pheromone-mediated G(1) arrest, we performed genetic and molecular experiments to test the possibility that Far3 participates in one of the heretofore characterized mechanisms, namely Fus3/Far1-mediated inhibition of Cdc28-Cln kinase activity, G(1) cyclin gene repression, and G(1) cyclin protein turnover. Our data indicate that Far3 effects G(1) arrest by a mechanism distinct from those previously known.
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