Analysis of Dominant Enhancers and Suppressors of Activated Notch in Drosophila

INVESTIGATIONS

Analysis of Dominant Enhancers and Suppressors of Activated Notch in Drosophila

E. M. Verheyen, K. J. Purcell, M. E. Fortini and S. Artavanis-Tsakonas
Howard Hughes Medical Institute and Departments of Cell Biology and Biology, Yale University, New Haven, Connecticut 06536

The Notch receptor controls cell fate decisions throughout Drosophila development. Truncated, ligand-independent forms of this protein delay or block differentiation. We have previously shown that expression of the intracellular domain of the receptor under the control of the sevenless enhancer/promoter induces a rough eye phenotype in the adult fly. Analysis of the resultant cellular transformations suggested that this form of Notch acts as a constitutively activated receptor. To identify gene products that interact with Notch, a second-site mutagenesis screen was performed to isolate enhancers and suppressors of the eye phenotype caused by expression of these activated Notch molecules. We screened 137,000 mutagenized flies and recovered 290 dominant modifiers. Many new alleles of previously identified genes were isolated, as were mutations defining novel loci that may function in the Notch signaling pathway. We discuss the data with respect to known features of Notch receptor signaling and Drosophila eye development.

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				M. Therrien, D. K. Morrison, A. M. Wong, and G. M. Rubin A Genetic Screen for Modifiers of a Kinase Suppressor of Ras-Dependent Rough Eye Phenotype in Drosophila Genetics, November 1, 2000; 156(3): 1231 - 1242. [Abstract] [Full Text]

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				A. Taguchi, K. Sawamoto, and H. Okano Mutations Modulating the Argos-Regulated Signaling Pathway in Drosophila Eye Development Genetics, April 1, 2000; 154(4): 1639 - 1648. [Abstract] [Full Text]

				I. Rebay, F. Chen, F. Hsiao, P. A. Kolodziej, B. H. Kuang, T. Laverty, C. Suh, M. Voas, A. Williams, and G. M. Rubin A Genetic Screen for Novel Components of the Ras/Mitogen-Activated Protein Kinase Signaling Pathway That Interact With the yan Gene of Drosophila Identifies split ends, a New RNA Recognition Motif-Containing Protein Genetics, February 1, 2000; 154(2): 695 - 712. [Abstract] [Full Text]

				N. I. Lukinova, V. V. Roussakova, and M. E. Fortini Genetic Characterization of Cytological Region 77A-D Harboring the Presenilin Gene of Drosophila melanogaster Genetics, December 1, 1999; 153(4): 1789 - 1797. [Abstract] [Full Text]

				A. C. Spradling, D. Stern, A. Beaton, E. J. Rhem, T. Laverty, N. Mozden, S. Misra, and G. M. Rubin The Berkeley Drosophila Genome Project Gene Disruption Project: Single P-Element Insertions Mutating 25% of Vital Drosophila Genes Genetics, September 1, 1999; 153(1): 135 - 177. [Abstract] [Full Text]

				K. Purcell and S. Artavanis-Tsakonas The Developmental Role of warthog, the Notch Modifier Encoding Drab6 J. Cell Biol., August 23, 1999; 146(4): 731 - 740. [Abstract] [Full Text] [PDF]

				S. Artavanis-Tsakonas, M. D. Rand, and R. J. Lake Notch Signaling: Cell Fate Control and Signal Integration in Development Science, April 30, 1999; 284(5415): 770 - 776. [Abstract] [Full Text]

				P zur Lage and A. Jarman Antagonism of EGFR and notch signalling in the reiterative recruitment of Drosophila adult chordotonal sense organ precursors Development, January 6, 1999; 126(14): 3149 - 3157. [Abstract] [PDF]

				G Daubresse, R Deuring, L Moore, O Papoulas, I Zakrajsek, W. Waldrip, M. Scott, J. Kennison, and J. Tamkun The Drosophila kismet gene is related to chromatin-remodeling factors and is required for both segmentation and segment identity Development, January 3, 1999; 126(6): 1175 - 1187. [Abstract] [PDF]

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				K Matsuno, M. Go, X Sun, D. Eastman, and S Artavanis-Tsakonas Suppressor of Hairless-independent events in Notch signaling imply novel pathway elements Development, January 11, 1997; 124(21): 4265 - 4273. [Abstract] [PDF]