- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Ewulonu, U. K.
- Articles by Schimenti, J.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Ewulonu, U. K.
- Articles by Schimenti, J.
Genetics, Vol 144, 785-792, Copyright © 1996
INVESTIGATIONS |
Targeted Mutagenesis of a Candidate t Complex Responder Gene in Mouse t Haplotypes Does Not Eliminate Transmission Ratio Distortion
U. K. Ewulonu, K. Schimenti, B. Kuemerle, T. Magnuson and J. Schimenti
The Jackson Laboratory, Bar Harbor, Maine 04609
Transmission ratio distortion (TRD) associated with mouse t haplotypes causes +/t males to transmit the t-bearing chromosome to nearly all their offspring. Of the several genes involved in this phenomenon, the t complex responder (Tcr(t)) locus is absolutely essential for TRD to occur. A candidate Tcr(t) gene called Tcp10b(t) was previously cloned from the genetically defined Tcr(t) region. Its location, restricted expression in testis, and a unique postmeiotic alternative splicing pattern supported the idea that Tcp10b(t) was Tcr(t). To test this hypothesis in a functional assay, ES cells were derived from a viable partial t haplotype, and the Tcp10b(t) gene was mutated by homologous recombination. Mutant mice were mated to appropriate partial t haplotypes to determine whether the targeted chromosome exhibited transmission ratios characteristic of the responder. The results demonstrated that the targeted chromosome retained full responder activity. Hence, Tcp10b(t) does not appear to be Tcr(t). These and other observations necessitate a reevaluation of genetic mapping data and the actual nature of the responder.
This article has been cited by other articles:
 |
|
 |
|
  G. J. Pazour, A. Koutoulis, S. E. Benashski, B. L. Dickert, H. Sheng, R. S. Patel-King, S. M. King, and G. B. Witman LC2, the Chlamydomonas Homologue of the t Complex-encoded Protein Tctex2, Is Essential for Outer Dynein Arm Assembly Mol. Biol. Cell, October 1, 1999; 10(10): 3507 - 3520. [Abstract] [Full Text]
|
|