- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Ahmad, K.
- Articles by Golic, K. G.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Ahmad, K.
- Articles by Golic, K. G.
Genetics, Vol 144, 657-670, Copyright © 1996
INVESTIGATIONS |
Somatic Reversion of Chromosomal Position Effects in Drosophila melanogaster
K. Ahmad and K. G. Golic
Department of Biology, University of Utah, Salt Lake City, Utah 84112
A transgene was inserted at several different chromosomal sites in Drosophila melanogaster, where its expression was subject to genomic position effects. Quantitative position effects and variegated and constant patterned position effects were observed. We investigated the status of the affected gene in the somatic cells where it normally functions. The FLP site-specific recombinase was used to remove the gene from the chromosome and its expression was then evaluated. We show that the FLP recombinase functions in cells that have finished their developmental program of mitoses. When FLP acts on directly repeated copies of its target site (FRT), the DNA flanked by those FRTs is excised from the chromosome as a closed circle. The extrachromosomal circle is maintained in nondividing cells, and a gene located on such a circle can be expressed. We then demonstrate that a gene subject to either variegated or constant position effect can be relieved of that effect by excision of the gene from the chromosome in cells where it would otherwise be inactive. We also observed a strong inhibition of FLP-mediated recombination for target sites located near centric heterochromatin.
This article has been cited by other articles:
 |
|
 |
|
  E. Brasset, F. Bantignies, F. Court, S. Cheresiz, C. Conte, and C. Vaury Idefix insulator activity can be modulated by nearby regulatory elements Nucleic Acids Res., April 10, 2007; (2007) gkm140v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ciapponi, G. Cenci, and M. Gatti The Drosophila Nbs Protein Functions in Multiple Pathways for the Maintenance of Genome Stability Genetics, July 1, 2006; 173(3): 1447 - 1454. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. P. Fitzgerald and W. Bender Polycomb Group Repression Reduces DNA Accessibility Mol. Cell. Biol., October 1, 2001; 21(19): 6585 - 6597. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T.-H. Cheng and M. R. Gartenberg Yeast heterochromatin is a dynamic structure that requires silencers continuously Genes & Dev., February 15, 2000; 14(4): 452 - 463. [Abstract] [Full Text]
|
|
|
|
|
|
Y. S. Rong and K. G. Golic Dominant Defects in Drosophila Eye Pigmentation Resulting From a Euchromatin-Heterochromatin Fusion Gene Genetics, December 1, 1998; 150(4): 1551 - 1566. [Abstract] [Full Text]
|
|
|
|
|
|
P. Liu, H. Zhang, A. McLellan, H. Vogel, and A. Bradley Embryonic Lethality and Tumorigenesis Caused by Segmental Aneuploidy on Mouse Chromosome 11 Genetics, November 1, 1998; 150(3): 1155 - 1168. [Abstract] [Full Text]
|
|
|
|
|
|
K. J. Beumer, S. Pimpinelli, and K. G. Golic Induced Chromosomal Exchange Directs the Segregation of Recombinant Chromatids in Mitosis of Drosophila Genetics, September 1, 1998; 150(1): 173 - 188. [Full Text]
|
|
|
|
 |
|
 T.-H. Cheng, Y.-C. Li, and M. R. Gartenberg Persistence of an alternate chromatin structure at silenced loci in the absence of silencers PNAS, May 12, 1998; 95(10): 5521 - 5526. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ahmad and K. G. Golic The Transmission of Fragmented Chromosomes in Drosophila melanogaster Genetics, February 1, 1998; 148(2): 775 - 792. [Abstract] [Full Text] [PDF]
|
|