A Test of the Double-Strand Break Repair Model for Meiotic Recombination in Saccharomyces cerevisiae

INVESTIGATIONS

A Test of the Double-Strand Break Repair Model for Meiotic Recombination in Saccharomyces cerevisiae

L. A. Gilbertson and F. W. Stahl
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403

We tested predictions of the double-strand break repair (DSBR) model for meiotic recombination by examining the segregation patterns of small palindromic insertions, which frequently escape mismatch repair when in heteroduplex DNA. The palindromes flanked a well characterized DSB site at the ARG4 locus. The ``canonical'' DSBR model, in which only 5' ends are degraded and resolution of the four-stranded intermediate is by Holliday junction resolvase, predicts that hDNA will frequently occur on both participating chromatids in a single event. Tetrads reflecting this configuration of hDNA were rare. In addition, a class of tetrads not predicted by the canonical DSBR model was identified. This class represented events that produced hDNA in a ``trans'' configuration, on opposite strands of the same duplex on the two sides of the DSB site. Whereas most classes of convertant tetrads had typical frequencies of associated crossovers, tetrads with trans hDNA were parental for flanking markers. Modified versions of the DSBR model, including one that uses a topoisomerase to resolve the canonical DSBR intermediate, are supported by these data.

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				L. Jessop, T. Allers, and M. Lichten Infrequent Co-conversion of Markers Flanking a Meiotic Recombination Initiation Site in Saccharomyces cerevisiae Genetics, March 1, 2005; 169(3): 1353 - 1367. [Abstract] [Full Text] [PDF]

				E. R. Hoffmann and R. H. Borts Trans Events Associated With Crossovers Are Revealed in the Absence of Mismatch Repair Genes in Saccharomyces cerevisiae Genetics, March 1, 2005; 169(3): 1305 - 1310. [Abstract] [Full Text] [PDF]

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				G. A. Cromie, C. A. Rubio, R. W. Hyppa, and G. R. Smith A Natural Meiotic DNA Break Site in Schizosaccharomyces pombe Is a Hotspot of Gene Conversion, Highly Associated With Crossing Over Genetics, February 1, 2005; 169(2): 595 - 605. [Abstract] [Full Text] [PDF]

				J. L. Argueso, J. Wanat, Z. Gemici, and E. Alani Competing Crossover Pathways Act During Meiosis in Saccharomyces cerevisiae Genetics, December 1, 2004; 168(4): 1805 - 1816. [Abstract] [Full Text] [PDF]

				E. C. Birmingham, S. A. Lee, R. D. McCulloch, and M. D. Baker Testing Predictions of the Double-Strand Break Repair Model Relating to Crossing Over in Mammalian Cells Genetics, November 1, 2004; 168(3): 1539 - 1555. [Abstract] [Full Text] [PDF]

				F. W. Stahl, H. M. Foss, L. S. Young, R. H. Borts, M. F. F. Abdullah, and G. P. Copenhaver Does Crossover Interference Count in Saccharomyces cerevisiae? Genetics, September 1, 2004; 168(1): 35 - 48. [Abstract] [Full Text] [PDF]

				A. Malkova, J. Swanson, M. German, J. H. McCusker, E. A. Housworth, F. W. Stahl, and J. E. Haber Gene Conversion and Crossing Over Along the 405-kb Left Arm of Saccharomyces cerevisiae Chromosome VII Genetics, September 1, 2004; 168(1): 49 - 63. [Abstract] [Full Text] [PDF]

				K. A. Borkovich, L. A. Alex, O. Yarden, M. Freitag, G. E. Turner, N. D. Read, S. Seiler, D. Bell-Pedersen, J. Paietta, N. Plesofsky, et al. Lessons from the Genome Sequence of Neurospora crassa: Tracing the Path from Genomic Blueprint to Multicellular Organism Microbiol. Mol. Biol. Rev., March 1, 2004; 68(1): 1 - 108. [Abstract] [Full Text] [PDF]

				L. R. Read, S. J. Raynard, A. Ruksc, and M. D. Baker Gene repeat expansion and contraction by spontaneous intrachromosomal homologous recombination in mammalian cells Nucleic Acids Res., February 20, 2004; 32(3): 1184 - 1196. [Abstract] [Full Text] [PDF]

				J. D. Merker, M. Dominska, and T. D. Petes Patterns of Heteroduplex Formation Associated With the Initiation of Meiotic Recombination in the Yeast Saccharomyces cerevisiae Genetics, September 1, 2003; 165(1): 47 - 63. [Abstract] [Full Text] [PDF]

				R. D. McCulloch, L. R. Read, and M. D. Baker Strand Invasion and DNA Synthesis From the Two 3' Ends of a Double-Strand Break in Mammalian Cells Genetics, April 1, 2003; 163(4): 1439 - 1447. [Abstract] [Full Text] [PDF]

				K. Y. Kwan, P. B. Moens, and J. C. Wang Infertility and aneuploidy in mice lacking a type IA DNA topoisomerase IIIbeta PNAS, March 4, 2003; 100(5): 2526 - 2531. [Abstract] [Full Text] [PDF]

				S. Palmer, E. Schildkraut, R. Lazarin, J. Nguyen, and J. A. Nickoloff Gene conversion tracts in Saccharomyces cerevisiae can be extremely short and highly directional Nucleic Acids Res., February 15, 2003; 31(4): 1164 - 1173. [Abstract] [Full Text] [PDF]

				E. R. Hoffmann, P. V. Shcherbakova, T. A. Kunkel, and R. H. Borts MLH1 Mutations Differentially Affect Meiotic Functions in Saccharomyces cerevisiae Genetics, February 1, 2003; 163(2): 515 - 526. [Abstract] [Full Text] [PDF]

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				P. B. Moens, N. K. Kolas, M. Tarsounas, E. Marcon, P. E. Cohen, and B. Spyropoulos The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination J. Cell Sci., April 15, 2002; 115(8): 1611 - 1622. [Abstract] [Full Text] [PDF]

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				J. Zalevsky, A. J. MacQueen, J. B. Duffy, K. J. Kemphues, and A. M. Villeneuve Crossing Over During Caenorhabditis elegans Meiosis Requires a Conserved MutS-Based Pathway That Is Partially Dispensable in Budding Yeast Genetics, November 1, 1999; 153(3): 1271 - 1283. [Abstract] [Full Text]

				K. J. Hillers and F. W. Stahl The Conversion Gradient at HIS4 of Saccharomyces cerevisiae. I. Heteroduplex Rejection and Restoration of Mendelian Segregation Genetics, October 1, 1999; 153(2): 555 - 572. [Abstract] [Full Text] [PDF]

				H. M. Foss, K. J. Hillers, and F. W. Stahl The Conversion Gradient at HIS4 of Saccharomyces cerevisiae. Genetics, October 1, 1999; 153(2): 573 - 583. [Abstract] [Full Text] [PDF]

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