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Genetics, Vol 143, 1785-1794, Copyright © 1996
INVESTIGATIONS |
Physical and Genetic Mapping of Chromosome 9S in Maize Using Mutations With Terminal Deficiencies
S. Chao, J. M. Gardiner, S. Melia-Hancock and E. H. Coe-Jr.
Department of Agronomy, University of Missouri, Columbia, Missouri 65211 Present address: Institute of Botany, Academia Sinica, Nankang, Taipei, Taiwan.
Deletion mapping was employed to determine the physical order of five morphological variants, pyd1, yg2, wd1, v28 and v31, with respect to restriction fragment length polymorphism (RFLP) markers located at the distal end of chromosome 9S in maize. The genetic materials used were a series of terminal-deficiency mutants, newly derived with MCCLINTOCK's original stocks developed in the 1940s, via break-age-fusion-bridge cycles. A combined physical map and genetic map has been constructed based on data gathered from both genetic complementation tests and RFLP analysis. The location of v31 in relation to RFLP markers was further determined by interval mapping. The physical distance between the healed telomeric end and the most distal RFLP marker in two terminal-deficiency lines was established by using pulsed field gel electrophoresis and verified by Bal31 digestion. The results from this study set a foundation for studies on the mechanism of healing of broken chromosome ends in higher plants.
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  Y.-Z. Zheng, R. R. Roseman, and W. R. Carlson Time Course Study of the Chromosome-Type Breakage-Fusion-Bridge Cycle in Maize Genetics, November 1, 1999; 153(3): 1435 - 1444. [Abstract] [Full Text]
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