- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Piruat, J. L.
- Articles by Aguilera, A.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Piruat, J. L.
- Articles by Aguilera, A.
Genetics, Vol 143, 1533-1542, Copyright © 1996
INVESTIGATIONS |
Mutations in the Yeast SRB2 General Transcription Factor Suppress hpr1-Induced Recombination and Show Defects in DNA Repair
J. L. Piruat and A. Aguilera
Departamento de Genetica, Facultad de Biologia, Universidad de Sevilla, E-41012 Sevilla, Spain
We have obtained genetic and molecular evidence that the hrs2-1 mutation, isolated as a suppressor of the hyperrecombination phenotype of hpr1{Delta}, is in the SRB2 gene, which encodes a component of the RNA polII holoenzyme. A newly constructed srb2{Delta} allele restores the wild-type levels of deletions in hpr1{Delta} cells, indicating that the lack of a functional SRB2 transcription factor suppresses recombination between direct repeats. These results suggest a direct connection between transcription and recombination between DNA repeats. On the other hand, the hrs2-1 mutation (renamed srb2-101), in which Gly(150) has been changed to Asp, makes cells sensitive to long MMS treatments, a phenotype observed for the srb2{Delta} null allele only in a hpr1{Delta} background. This indicates that mutations in the basal transcription factor SRB2 impair DNA repair of MMS-induced damage, which adds a new connection between transcription and DNA repair. We discuss the possibility that hpr1-induced deletions occurred as a consequence of a SRB2-dependent stalled or blocked transcription complex.
This article has been cited by other articles:
 |
|
 |
|
  B. Palancade, X. Liu, M. Garcia-Rubio, A. Aguilera, X. Zhao, and V. Doye Nucleoporins Prevent DNA Damage Accumulation by Modulating Ulp1-dependent Sumoylation Processes Mol. Biol. Cell, August 1, 2007; 18(8): 2912 - 2923. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Malagon, A. H. Tong, B. K. Shafer, and J. N. Strathern Genetic Interactions of DST1 in Saccharomyces cerevisiae Suggest a Role of TFIIS in the Initiation-Elongation Transition Genetics, March 1, 2004; 166(3): 1215 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Chang, M. Bellaoui, C. Boone, and G. W. Brown A genome-wide screen for methyl methanesulfonate-sensitive mutants reveals genes required for S phase progression in the presence of DNA damage PNAS, December 24, 2002; 99(26): 16934 - 16939. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. J. Merker and H. L. Klein Role of Transcription in Plasmid Maintenance in the hpr1{Delta} Mutant of Saccharomyces cerevisiae Mol. Cell. Biol., December 15, 2002; 22(24): 8763 - 8773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Merker and H. L. Klein hpr1{Delta} Affects Ribosomal DNA Recombination and Cell Life Span in Saccharomyces cerevisiae Mol. Cell. Biol., January 15, 2002; 22(2): 421 - 429. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-Y. Fan, R. J. Merker, and H. L. Klein High-Copy-Number Expression of Sub2p, a Member of the RNA Helicase Superfamily, Suppresses hpr1-Mediated Genomic Instability Mol. Cell. Biol., August 15, 2001; 21(16): 5459 - 5470. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. D. Parvin BRCA1 at a branch point PNAS, May 22, 2001; 98(11): 5952 - 5954. [Full Text] [PDF]
|
|
|
|
|
|
R. Schneiter, C. E. Guerra, M. Lampl, G. Gogg, S. D. Kohlwein, and H. L. Klein The Saccharomyces cerevisiae Hyperrecombination Mutant hpr1Delta Is Synthetically Lethal with Two Conditional Alleles of the Acetyl Coenzyme A Carboxylase Gene and Causes a Defect in Nuclear Export of Polyadenylated RNA Mol. Cell. Biol., May 1, 1999; 19(5): 3415 - 3422. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Hampsey Molecular Genetics of the RNA Polymerase II General Transcriptional Machinery Microbiol. Mol. Biol. Rev., June 1, 1998; 62(2): 465 - 503. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. C. Myers, C. M. Gustafsson, D. A. Bushnell, M. Lui, H. Erdjument-Bromage, P. Tempst, and R. D. Kornberg The Med proteins of yeast and their function through the RNA polymerase II carboxy-terminal domain Genes & Dev., January 1, 1998; 12(1): 45 - 54. [Abstract] [Full Text]
|
|
|
|
|
|
S. Chavez and A. Aguilera The yeast HPR1 gene has a functional role in transcriptional elongation that uncovers a novel source of genome instability Genes & Dev., December 15, 1997; 11(24): 3459 - 3470. [Abstract] [Full Text] [PDF]
|
|