- THIS ARTICLE
- Full Text (PDF)
- Alert me when this article is cited
- Alert me if a correction is posted
- SERVICES
- Similar articles in this journal
- Similar articles in PubMed
- Alert me to new issues of the journal
- Download to citation manager
- Reprints & Permissions
- CITING ARTICLES
- Citing Articles via HighWire
- Citing Articles via Google Scholar
- GOOGLE SCHOLAR
- Articles by Miller, L. M.
- Articles by Kim, S. K.
- Search for Related Content
- PUBMED
- PubMed Citation
- Articles by Miller, L. M.
- Articles by Kim, S. K.
Genetics, Vol 143, 1181-1191, Copyright © 1996
INVESTIGATIONS |
Mosaic Analysis Using a ncl-1 (+) Extrachromosomal Array Reveals That lin-31 Acts in the Pn.p Cells During Caenorhabditis elegans Vulval Development
L. M. Miller, D. A. Waring and S. K. Kim
Department of Developmental Biology, Stanford University Medical Center, Stanford, California 94305 Present address: Department of Biology, Santa Clara University, Santa Clara, CA 95053.
We describe a genetic mosaic analysis procedure in which Caenorhabditis elegans mosaics are generated by spontaneous loss of an extrachromosomal array. This technique allows almost any C. elegans gene that can be used in germline transformation experiments to be used in mosaic analysis experiments. We identified a cosmid clone that rescues the mutant phenotype of ncl-1, so that this cell-autonomous marker could be used to analyze mosaic animals. To determine the sites of action for unc-29 and lin-31, an extrachromosomal array was constructed containing the ncl-1(+) cosmid linked to lin-31(+) and unc-29(+) cosmids. This array is mitotically unstable and can be lost to produce a clone of mutant cells. The specific cell division at which the extrachromosomal array had been lost was deduced by scoring the Ncl phenotypes of individual cells in genetic mosaics. The Unc-29 and Lin-31 phenotypes were then scored in these animals to determine in which cells these genes are required. This analysis showed that unc-29, which encodes a subunit of the acetylcholine receptor, acts in the body muscle cells. Furthermore, lin-31, which specifies cell fates during vulval induction and encodes a putative transcription factor similar to HNF-3/fork head, acts in the Pn.p cells.
This article has been cited by other articles:
 |
|
 |
|
  J. Yochem and R. K. Herman Investigating C. elegans development through mosaic analysis Development, October 15, 2003; 130(20): 4761 - 4768. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Ohkura, N. Suzuki, T. Ishihara, and I. Katsura SDF-9, a protein tyrosine phosphatase-like molecule, regulates the L3/dauer developmental decision through hormonal signaling in C. elegans Development, July 15, 2003; 130(14): 3237 - 3248. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Sanford and M. D. Perry Asymmetrically distributed oligonucleotide repeats in the Caenorhabditis elegans genome sequence that map to regions important for meiotic chromosome segregation Nucleic Acids Res., July 15, 2001; 29(14): 2920 - 2926. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. M. Miller, H. A. Hess, D. B. Doroquez, and N. M. Andrews Null Mutations in the lin-31 Gene Indicate Two Functions During Caenorhabditis elegans Vulval Development Genetics, December 1, 2000; 156(4): 1595 - 1602. [Abstract] [Full Text]
|
|
|
|
|
|
J. Yochem, T. Gu, and M. Han A New Marker for Mosaic Analysis in Caenorhabditis elegans Indicates a Fusion Between hyp6 and hyp7, Two Major Components of the Hypodermis Genetics, July 1, 1998; 149(3): 1323 - 1334. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. J. Frank and M. B. Roth ncl-1 Is Required for the Regulation of Cell Size and Ribosomal RNA Synthesis in Caenorhabditis elegans J. Cell Biol., March 23, 1998; 140(6): 1321 - 1329. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Eisenmann, J. Maloof, J. Simske, C Kenyon, and S. Kim The beta-catenin homolog BAR-1 and LET-60 Ras coordinately regulate the Hox gene lin-39 during Caenorhabditis elegans vulval development Development, January 9, 1998; 125(18): 3667 - 3680. [Abstract] [PDF]
|
|
|
|
|
|
Analysis of osm-6, a Gene That Affects Sensory Cilium Structure and Sensory Neuron Function in Caenorhabditis elegans Genetics, January 1, 1998; 148(1): 187 - 200.
|
|
|
|
 |
|
 A. Hajnal, C. W. Whitfield, and S. K. Kim Inhibition of Caenorhabditis elegans vulval induction by gap-1 and by let-23 receptor tyrosine kinase Genes & Dev., October 15, 1997; 11(20): 2715 - 2728. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. T. Fleming, M. D. Squire, T. M. Barnes, C. Tornoe, K. Matsuda, J. Ahnn, A. Fire, J. E. Sulston, E. A. Barnard, D. B. Sattelle, et al. Caenorhabditis elegans Levamisole Resistance Genes lev-1, unc-29, and unc-38 Encode Functional Nicotinic Acetylcholine Receptor Subunits J. Neurosci., August 1, 1997; 17(15): 5843 - 5857. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Bettinger, S Euling, and A. Rougvie The terminal differentiation factor LIN-29 is required for proper vulval morphogenesis and egg laying in Caenorhabditis elegans Development, January 11, 1997; 124(21): 4333 - 4342. [Abstract] [PDF]
|
|