The Drosophila ash1 Gene Product, Which Is Localized at Specific Sites on Polytene Chromosomes, Contains a SET Domain and a PHD Finger

INVESTIGATIONS

The Drosophila ash1 Gene Product, Which Is Localized at Specific Sites on Polytene Chromosomes, Contains a SET Domain and a PHD Finger

N. Tripoulas, D. LaJeunesse, J. Gildea and A. Shearn
Present address: Department of Molecular Biology, The Cleveland Clinic Foundation, Cleveland, OH 44195.

The determined state of Drosophila imaginal discs depends on stable patterns of homeotic gene expression. The stability of these patterns requires the function of the ash1 gene, a member of the trithorax group. The primary translation product of the 7.5-kb ash1 transcript is predicted to be a basic protein of 2144 amino acids. The ASH1 protein contains a SET domain and a PHD finger. Both of these motifs are found in the products of some trithorax group and Polycomb group genes. We have determined the nucleotide sequence alterations in 10 ash1 mutant alleles and have examined their mutant phenotype. The best candidate for a null allele is ash1(22). The truncated protein product of this mutant allele is predicted to contain only 47 amino acids. The ASH1 protein is localized on polytene chromosomes of larval salivary glands at >100 sites. The chromosomal localization of ASH1 implies that it functions at the transcriptional level to maintain the expression pattern of homeotic selector genes.

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				T. Rozovskaia, S. Tillib, S. Smith, Y. Sedkov, O. Rozenblatt-Rosen, S. Petruk, T. Yano, T. Nakamura, L. Ben-Simchon, J. Gildea, et al. Trithorax and ASH1 Interact Directly and Associate with the Trithorax Group-Responsive bxd Region of the Ultrabithorax Promoter Mol. Cell. Biol., September 1, 1999; 19(9): 6441 - 6447. [Abstract] [Full Text] [PDF]

				S. Tillib, S. Petruk, Y. Sedkov, A. Kuzin, M. Fujioka, T. Goto, and A. Mazo Trithorax- and Polycomb-Group Response Elements within an Ultrabithorax Transcription Maintenance Unit Consist of Closely Situated but Separable Sequences Mol. Cell. Biol., July 1, 1999; 19(7): 5189 - 5202. [Abstract] [Full Text] [PDF]

				T. R. Breen Mutant Alleles of the Drosophila trithorax Gene Produce Common and Unusual Homeotic and Other Developmental Phenotypes Genetics, May 1, 1999; 152(1): 319 - 344. [Abstract] [Full Text]

				M Vazquez, L Moore, and J. Kennison The trithorax group gene osa encodes an ARID-domain protein that genetically interacts with the brahma chromatin-remodeling factor to regulate transcription Development, January 2, 1999; 126(4): 733 - 742. [Abstract] [PDF]

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