Genetics, Vol 143, 877-885, Copyright © 1996

INVESTIGATIONS

A Novel Drosophila Minute Locus Encodes Ribosomal Protein S13

S. S{aelig}b{oslash}e-Larssen and A. Lambertsson
Department of Biology, Division of General Genetics, University of Oslo, N-0315 Oslo, Norway

Minutes comprise >50 phenotypically similar Drosophila mutations believed to affect ribosomal protein genes. Common traits of the Minute phenotype are short and thin bristles, slow development, and recessive lethality. To further investigate the proposed Minute to ribosomal protein correspondence, loss-of-function Minute mutations were induced by P-element mutagenesis. Here, we report a previously undescribed Minute locus that maps to 32A on chromosome 2L; this Minute allele is named P{lac-W}M(2)32A(1) and the gene M(2)32A. Flies heterozygous for P{lacW}M(2)32A(1) have a medium Minute phenotype. The gene interrupted by the P-element insertion was cloned. Sequence analyses revealed that it encodes the Drosophila homologue of eukaryotic ribosomal protein S13. It is a single-copy gene and the level of RPS13 transcript is reduced to ~50% in P{lacW}M(2)32A(1) heterozygotes. Both transcript level and phenotype are restored to wild type by remobilizing the P element, demonstrating that the mutation is caused by insertion of the P-element construct. These results further strengthen the notion that Minutes encode ribosomal proteins and demonstrate that P-element mutagenesis is a fruitful approach to use in these studies.

This article has been cited by other articles:

				D. Yamashita, Y. Sano, Y. Adachi, Y. Okamoto, H. Osada, T. Takahashi, T. Yamaguchi, T. Osumi, and F. Hirose hDREF Regulates Cell Proliferation and Expression of Ribosomal Protein Genes Mol. Cell. Biol., March 15, 2007; 27(6): 2003 - 2013. [Abstract] [Full Text] [PDF]

				S. R. Schulze, D. A. R. Sinclair, K. A. Fitzpatrick, and B. M. Honda A Genetic and Molecular Characterization of Two Proximal Heterochromatic Genes on Chromosome 3 of Drosophila melanogaster Genetics, April 1, 2005; 169(4): 2165 - 2177. [Abstract] [Full Text] [PDF]

				C. Martin-Castellanos and B. A. Edgar A characterization of the effects of Dpp signaling on cell growth and proliferation in the Drosophila wing Development, March 4, 2003; 129(4): 1003 - 1013. [Abstract] [Full Text] [PDF]

				D. Weijers, M. Franke-van Dijk, R.-J. Vencken, A. Quint, P. Hooykaas, and R. Offringa An Arabidopsis Minute-like phenotype caused by a semi-dominant mutation in a RIBOSOMAL PROTEIN S5 gene Development, November 1, 2001; 128(21): 4289 - 4299. [Abstract] [Full Text] [PDF]

				A ION, A H CROSBY, H KREMER, N KENMOCHI, M VAN REEN, C FENSKE, I VAN DER BURGT, H G BRUNNER, K MONTGOMERY, R S KUCHERLAPATI, et al. Detailed mapping, mutation analysis, and intragenic polymorphism identification in candidate Noonan syndrome genes MYL2, DCN, EPS8, and RPL6 J. Med. Genet., November 1, 2000; 37(11): 884 - 886. [Full Text]

				I. Torok, D. Herrmann-Horle, I. Kiss, G. Tick, G. Speer, R. Schmitt, and B. M. Mechler Down-Regulation of RpS21, a Putative Translation Initiation Factor Interacting with P40, Produces Viable Minute Imagos and Larval Lethality with Overgrown Hematopoietic Organs and Imaginal Discs Mol. Cell. Biol., March 1, 1999; 19(3): 2308 - 2321. [Abstract] [Full Text] [PDF]

				N. Kenmochi, T. Kawaguchi, S. Rozen, E. Davis, N. Goodman, T. J. Hudson, T. Tanaka, and D. C. Page A Map of 75 Human Ribosomal Protein Genes Genome Res., May 1, 1998; 8(5): 509 - 523. [Abstract] [Full Text]

				S. Saboe-Larssen, M. Lyamouri, J. Merriam, M. P. Oksvold, and A. Lambertsson Ribosomal Protein Insufficiency and the Minute Syndrome in Drosophila: A Dose-Response Relationship Genetics, March 1, 1998; 148(3): 1215 - 1224. [Abstract] [Full Text] [PDF]