Genetics, Vol 143, 713-721, Copyright © 1996

INVESTIGATIONS

Destabilization of Simple Repetitive DNA Sequences by Transcription in Yeast

M. Wierdl, C. N. Greene, A. Datta, S. Jinks-Robertson and T. D. Petes
Department of Biology and Curriculum in Genetics and Molecular Biology, University of North Carolina, Chapel Hill, North Carolina 27599-3280

Simple repetitive DNA sequences in the eukaryotic genome frequently alter in length. In wild-type strains, we find that transcription through a repetitive poly GT tract destabilizes the tract four- to ninefold. In mismatch repair-deficient yeast strains, simple repeats are very unstable. High levels of transcription in such strains destabilize repetitive tracts an additional two- to threefold.

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